贵阳市无偿献血人群新布尼亚病毒血清流行病学调查

An investigation on the serum epidemiology of the new-Bunyavirus among the blood-donors in Guiyang city

目的分析新布尼亚病毒在贵阳无偿献血人群中的潜在感染或既往感染情况。方法收集2015-2016年贵州省血液中心无偿献血人群血浆标本1 187份进行ELISA重复检测,使用Excel2007软件对所有标本基本资料进行录入整理,并采用SPSS 19.0软件对不同性别、年龄、职业、地区、采集时间及民族来源的标本进行统计学分析。结果 1 187份标本中,阳性标本共63份,在贵阳市无偿献血人群中新布尼亚病毒血清总抗体阳性率为5.31%,63份阳性标本总抗体效价为1∶2~1∶64。新布尼亚病毒抗体阳性率在不同性别、年龄、职业、地区、民族之间比较,差异均无统计学意义(P>0.05),而流行期与流行后期采集的标本阳性率比较,差异有统计学意义(χ~2=4.623,P=0.032)。总抗体阳性标本进行核酸检测,结果均为阴性。结论贵阳市无偿献血人群中存在新布尼亚病毒总抗体阳性标本,提示贵阳市无偿献血人群存在新布尼亚病毒感染的可能。

Objective To investigate the potential infectious of the new-Bunyavirus among the blood-donors in Guiyang area.Methods A total of 1 187 blood samples and the basic data were collected from the blood center of Guizhou.The new-Bunyavirus total antibody in serum was tested by enzyme-linked immunosorbent assay（ELISA）.The samples were tested repeatedly by ELSIA,and general statistics description of the samples′ data was did by Excel2007,and the age,gender,occupation and collecting time of the samples were analyzed by SPSS19.0.Results The positive rate of new-Bunyavirus total antibody among blood-donors in Guiyang was 5.31%,and the new-Bunyavirus total antibody titers was 1∶20-1∶64.The positive were tested by PCR for identifying,PCR results of all positive samples were negative.The positive rates between different genders,ages,occupations,and nationalities were not statistically significant（P〉0.05）,but the difference of positive rates between epidemic period and later stages of the epidemic was statistically significant（χ2=4.623,P=0.032）.Conclusion There are new-Bunyavirus total antibody positive samples in blood-donors,noticing that there is a possibility of potential infection among Guiyang blood-donors and even healthy people.