大豆生长素受体基因家族及组织化学表达模式分析

Analysis of soybean auxin receptor gene family and histochemical expression pattern

生长素是调控植物生长的主要激素之一,TIR1/AFB是主要生长素受体基因,参与生长素信号的响应及传递。本研究分析了大豆中生长素受体TIR1/AFB基因家族的各个成员,依据拟南芥生长素受体基因序列比对及系统进化分析,可将大豆生长素受体分为三个聚类,其中I类和II类分别作为ATTIR1/AFB1和ATAFB2/AFB3的同源蛋白均含有FBOX和LRR(Leucine Rich Repeat)结构域,III类作为ATAFB4/AFB5的同源蛋白仅含LC(Low complexity)结构域。基因表达芯片分析结果表明,部分大豆生长素受体基因在不同组织中具有特异性的表达模式,预示着这些基因可能参与相关生物学过程的调控。组织化学表达模式检测结果表明,部分生长素受体在大豆主根及侧根中具有特异表达模式,预示这些基因可能参与大豆主根生长及侧根发生的调控过程。

Auxin is one of the most important hormones regulating plant growth and development,and TIR1/AFBs are the main auxin receptors involved in auxin response and auxin signaling pathway. In this study,we analyzed TIR1/AFB family members in soybean. According to the phylogenetic analysis of auxin receptors between Arabidopsis and soybean,the soybean TIR1/AFB genes could be subdivided into three groups： clade I and clade II gene members being orthologs of ATTIR1/AFB1 and ATAFB2/AFB3. All of those proteins contain FBOX and leucine rich repeat domain. The clade III gene members were orthologs of ATAFB4/AFB5,containing low complexity structure. The transcriptome data showed that some of soybean auxin receptors performed tissue specific expression patterns,indicating that those genes may function in related development stage. Histochemical staining results showed that some of the soybean auxin receptors expressed specifically in root tips and lateral root primordia,suggesting the important roles of those genes in regulation of soybean main root development and lateral root growth.