α-硫辛酸对糖调节受损大鼠认知功能和氧化应激影响的初步研究

A preliminary study of the effects of α-lipoic acid on cognitive function and oxidative stress in rats with impaired glucose regulation

目的研究硫辛酸对糖调节受损大鼠认知功能和氧化应激的影响。方法雄性Wistar大鼠随机分为5组,对照组、模型组、硫辛酸低[15 mg/(kg·d)]、中[30 mg/(kg·d)]、高[60 mg/(kg·d)]剂量组。用高脂高糖饲料饲养法建立糖调节受损大鼠模型,给予成功的大鼠模型连续每日灌胃硫辛酸溶液15 mg/kg、30 mg/kg、60 mg/kg,每日一次,共14 d。用Morris水迷宫法检测大鼠学习记忆能力;用流式细胞仪检测各组大鼠海马组织的ROS含量值。结果与对照组相比,模型组大鼠学习记忆能力下降(P<0.05),海马组织内ROS水平升高(P<0.05);与模型组相比,硫辛酸组脑内ROS水平下降,大鼠的学习和记忆能力提高,尤以中剂量组改变明显(P<0.05)。结论α-硫辛酸对糖调节受损大鼠的认知功能有保护作用,该保护作用与其抗氧化作用有关。

Objective To study the effects of lipoic acid on cognitive function and oxidative stress in rats with impaired glucose regulation. Methods Male Wistar rats were randomly divided into 5 groups： control group,model group,lipoic acid low 15 mg/（ kg/d）,medium 30 mg/（ kg/d）,high 60 mg/（ kg/d） dose group. A rat model of impaired glucose regulation was established by feeding with high fat and high sugar diet. The model rats were fed with lipoic acid solution 15,30 and 60 mg/kg daily,1 times daily,with a total of 14 d. The learning and memory abilities of rats were measured by Morris water maze method,and the content of ROS in hippocampus of rats in each group was detected by flow cytometry. Results Compared with the control group,the learning and memory ability of the model group decreased（ P〈0. 05）,while the level of ROS in the hippocampus increased（ P〈0. 05）. Compared with the model group,the level of ROS in the brain decreased in the lipoic acid group,and the learning and memory ability of the rats increased,especially in the middle dose group（ P〈0. 05）. Conclusion Lipoic acid has protective effects on cognitive function in rats with impaired glucose regulation,and its protective effects are related to its antioxidant effects.