申克孢子丝菌chs1基因生物信息分析

Bioinformatics analysis of chs1 gene from Sporothrix schenckii

目的:对申克孢子丝菌几丁质合成酶基因(chs1)及其编码蛋白的结构和特性进行分析和预测。方法:利用NCBI网站、Ex PASy和CBS等工具预测申克孢子丝菌chs1基因及编码蛋白的结构和功能。结果:chs1 mRNA全长2 745 bp,编码914个氨基酸。BLAST分析显示,申克孢子丝菌与巴西孢子丝菌、蓝菌属真菌、足马杜拉分枝菌等真菌chs氨基酸序列一致性达到99%,且有保守域。预测chs1蛋白相对分子量为102 714.7,理论等电点为6.71。预测chs1编码蛋白ɑ螺旋、β折叠、β转角、无规则卷的比例分别是38.40%、17.94%、10.39%、33.26%。chs1蛋白是一种疏水蛋白,包含9个跨膜区,未发现信号肽。结论:成功预测了chs1基因及编码蛋白质的生化性质及结构特征,为下一步对其进行克隆、表达和敲除奠定基础。

Objective： To analyze the structure and characteristics of Chitin synthase 1 gene （ chsl） and its coding protein of Sporothrix schenckii by bioinformatics. Metliods ： The mRNA se-quence of chs1 was found on the NCBI web site, and the charactemstics of its coding protein wasanalyzed by professional online tools from ExPASy,CBS and so on. Results：The full length of chs1 mRNA is 2 745 bp, encodes 914 amino acids,and the identity of its sequence was up to 99%compared to other fungi. According to the prediction,chs1 protein had conserved domain, its mo-lecular weight was 102 714. 7 ,and the theoretical pi was 6. 71. The proporion of tended strand, p - tur and random coil were 38.40% , 17. 94% , 10. 39% , 33. 26% respective-ly. chs1 protein was a kind of hydrophobic protein which contained nine transmembraneregions and none signalpeptide. Conclusion： The structure and characteristics of Ss. chsl and its proteinhave been predicted.