Nrf2-Keap1系统及相关因子在小鼠黄褐斑组织中的表达

Expression of Nrf2-Keap1 system and related factors in mouse melasma

目的:探讨核因子E2相关因子2(Nrf2)-Kelch样环氧氯丙烷相关蛋白1(Keap1)系统及相关因子在小鼠黄褐斑模型皮肤中的变化。方法:将24只Balb/c雌性小鼠随机分成2组,每组12只。模型组采用中波紫外线照射联合黄体酮注射方法造模,对照组肌肉注射同剂量的灭菌注射用水,采用q PCR相对定量检测小鼠皮肤的Nrf2和Keap1基因表达水平,化学比色法检测小鼠皮肤中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与对照组相比,模型组小鼠皮肤组织的Keap1 mRNA表达量明显增强(P<0.01),Nrf2 mRNA表达量无明显改变(P>0.05),模型组SOD和GSH-Px活性明显下降(P<0.01),MDA含量明显上升(P<0.01)。结论:氧化应激在黄褐斑发病中起着非常重要的作用,抗氧化损伤Nrf2-Keap1系统与黄褐斑的发病关系密切,可为黄褐斑的治疗提供新的靶点。

Objective: To investigate the effects of nuclear factor-E2-related 2 factor( Nrf2)-Kelch-like ECH-associated 1 protein( Keap1) system and related factors change in the skin of the mouse model of chloasma. Methods: Twenty-four Balb/c female mice were randomly divided into 2 groups,12 in each group. The mice in the model group were treated with UVB irradiation combined with progesterone injection. The mice in the control group were injected with the same dose of sterile water for injection. The expression of NRF2 and Keap1 gene in the skin of mice was detected by q PCR. The contents of malondialdehyde( MDA) and the activities of superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px) in the skin of mice were detected by chemical colorimetric assay. Results: Compared with the control group,the expression of Keap1 mRNA in the skin tissue of the model group was significantly increased( P < 0. 01),however,the expression of Nrf2 mRNA did not change significantly( P > 0. 05). The activities of SOD and GSHPx were significantly decreased( P < 0. 01),and the content of MDA increased significantly( P <0. 01) in the skin tissue of the model group. Conclusion: Oxidative stress plays a very important role in the pathogenesis of chloasma. The Nrf2-Keap1 system is closely related to the pathogenesis of chloasma,which provides a new target for the treatment of chloasma.