建立检测制式长枪上DNA多态性的新方法

A new methodology of DNA polymorphism detection from standard long gun

目的建立快速、准确检测制式长枪上基因分型的新方法。方法联合使用直扩法、硅膜法对48支制式长枪上5个不同部位共240份检材进行DNA检测。结果联合使用直扩法及硅膜法,在48支制式长枪中检测出DNA完整分型42支,检出率达87.50%。结论联合使用直扩法、硅膜法,可有效快速、准确地检测出制式长枪上DNA多态性。

Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun.