骨髓间充质干细胞移植对光气吸入性肺损伤大鼠肺组织及血浆炎症因子的干预作用

The intervention effect of bone marrow-derived mesenchymal stem cells on inflammatory factors in lung tissue and plasma of phosgene inhalation lung injury rats

目的:观察骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,BMD-MSCs)移植对光气吸入性肺损伤大鼠肺组织及血浆炎症因子的干预作用。方法:96只雄性SD大鼠随机分为:空气对照组(A组)、BMD-MSCs干预对照组(AM组)、光气染毒组(PH组)、光气染毒后BMD-MSCs干预组(PM组),每组8只。染毒组按8.33 mg/L动态恒量染毒5min。PM组染毒后即刻经尾静脉注射10~6 BMD-MSCs。4组分别于模型完成后6、24、48h处死大鼠,取肺组织行病理检查及肺湿/干比、血气分析,取血浆行Bio-Plex细胞因子检测。结果:PH组较A组TNF-α浓度明显升高(P<0.05);PM组较PH组TNF-α浓度降低(P<0.05)。PM组与PH组血浆IL-4浓度6h、24h均降低,以PM组下降更明显(P<0.05);48h明显升高,以PM组升高更明显(P<0.05)。PH组较A组IL-10浓度高(P<0.05);PM组较PH组IL-10浓度高(P=0.013)。结论:经尾静脉注射BMD-MSCs后可以使大鼠血浆中抗炎因子IL-4和IL-10逐渐升高,促炎因子TNF-α逐渐下降,提示其对光气致急性肺损伤大鼠肺具有保护作用。

Objective：To observe the intervention effect of bone marrow-derived mesenchymal stem cells （BMD-MSCs） on inflammatory factors in lung tissue and plasma of phosgene inhalation lung injury rats.Methods：96 male SD rats were randomly divided into air control group （group A）, BMD-MSCs intervention control group （group AM）, phosgene exposure group （group PH） and BMD-MSCs intervention group after phosgene exposure （group PM）, with 8 rats in each group.Group PH was exposed to dynamic constant phosgene at 8.33 mg/L for 5 min.Group PM was injected with 106 BMD-MSCs via the tail vein immediately after phosgene exposure.The rats in the 4 groups were sacrificed at 6, 24 and 48 h after the models were completed.The lung tissues were taken for pathological examination, lung wet / dry ratio and blood gas analysis.The plasma was taken for the Bio-Plex cytokine test.Results：The level of TNF-α was higher in group PH than group A, and the difference was statistically significant （P=0.02）.The level of TNF-α was lower in the group PM than group PH （P〈0.05）.The plasma IL-4 concentration in group PM and group PH decreased by 6h and 24 h, which was more significant in group PM （P〈0.05）.It increased significantly by 48h, which was more significant in group PM （P〈0.05）.The level of IL-10 was higher in group PM than group PH, and the difference was statistically significant （P〈0.05）.The level of IL-10 was higher in group PM than group PH, and the difference was statistically significant （P〈0.05）.Conclusions：Anti-inflammatory cytokines IL-10 and IL-4 were increased and pro-inflammatory cytokines TNF-α was decreased by the tail vein injection of BMD-MSCs, so BMD-MSCs has a protective effect in rats with acute lung injury induced by phosgene.