银纳米粒子光度法测定药品中培氟沙星的含量

Photometric determination of pefloxacin content in drug by Silver Nanoparticles

目的建立药品中培氟沙星的银纳米粒子光度测定法。方法在10 ml比色管中,依次加入2.5 ml银纳米溶液和一定量的培氟沙星标准溶液,1.0 ml p H值为6.09三酸缓冲溶液,用超纯水定容至刻度室温反应20 min后,测定体系400 nm处的吸收度。结果培氟沙星在0.4μg/ml^1.5μg/ml时与银纳米粒子400 nm处的吸光度值呈良好的线性关系,线性回归方程为A_0/A=0.283 8C+0.709 7,相关系数r=0.995 0,方法检出限为0.063μg/ml,平均回收率为91.5%~94.8%。结论银纳米粒子光度测定培氟沙星的方法具有快速简便,灵敏度和精密度较高的优点,适用于药品中培氟沙星的检测。

Objective To develop a novel method for the determination of pefloxacin based on the photometry of Silver Nanoparticles（ Ag NPs）. Methods In colorimetric tube,2. 5 ml Ag NPs solution,a certain amount of pefloxacin standard solution,and1 ml of B-R buffer medium of p H 6. 09 were added. The solution was diluted to 10 ml with ultrapure water,then reacted at room temperature for 20 min. The absorbance of pefloxacin was determined at 400 nm. Results The linear relationship between relative absorbance of perfloxacin at 400 nm and concentration of pefloxacin kept well in the range of 0. 4 μg/ml-1. 5μg/ml. The linear regression equation A_0/A = 0. 283 8C ＋ 0. 709 7,the correlation coefficient（ r） was 0. 995 0. The detection limit was 0. 063 μg/ml and the recovery rates were within 91. 5%-94. 8%. Conclusion This method is sensitive,accurate and it is suitable for the determination of pefloxacin in drug samples with the satisfactory results.