肝细胞肝癌患者ZHX2、GPC3、H19基因表达水平及其相关关系

The expression levels of ZHX2,GPC3,H19 mRNA in hepatocellular carcinoma and its relationship to clinicopathologic features

目的研究舟山海岛地区肝细胞肝癌(HCC)组织与相应癌旁肝组织新型转录抑制因子锌指和同源框2(ZHX2)基因、磷脂酰肌醇蛋白聚糖-3(GPC3)mRNA和长链非编码RNA(IncRNA)H19基因的表达差异及其与临床病理特征的关系。方法收集17例原发性肝癌患者手术切除肿瘤组织及相应癌旁肝组织,反转录实时荧光定量PCR(qRTPCR)检测ZHX2、GPC3 mRNA和H19基因表达,并分析其与临床病理特征的关系。结果 ZHX2基因mRNA在HCC中表达明显低于癌旁肝组织,而GPC3基因mRNA在HCC中表达则明显高于癌旁肝组织,差异有统计学意义(P<0.05)。HCC患者ZHX2 mRNA表达与GPC3呈负相关,GPC3mRNA表达与血清AFP水平呈正相关,差异有统计学意义(r=-0.626 8,r=0.536 7,P<0.05),而且GPC3 mRNA表达增高患者多伴有乙型肝炎病毒感染(P<0.05)。结论肝细胞肝癌组织中ZHX2与GPC3具有明显相关性,ZHX2可能通过调控GPC3参与肝细胞肝癌的发生发展。

Objective To explore the different expression levels of zinc-fingers and homeoboxes 2（ ZHX2）,glypican-3（ GPC3）,and IncRNA H19 in patients with hepatocellular carcinoma（ HCC） in Zhoushan Achipelog and its relationship with clinical pathological features. Methods The expression levels of ZHX2,GPC3 mRNA and H19 were detected by reverse transcriptional real-time fluorescent quantitative PCR（ qRT-PCR） in 17 paired HCC tumor tissue and adjacent liver tissues,and the correlation with clinic-pathological features were analyzed. Results The expression levels of ZHX2 mRNA were obviously lower in HCC than those in adjacent liver tissue,whereas the expression levels of GPC3 mRNA were significantly higher in HCC than those in adjacent liver tissue,with the differences statistically significant（ P〈 0. 05）. The expression levels of ZHX2 mRNA were negatively correlated with the levels of GPC3 mRNA,which were positively correlated with serum level of alpha fetoprotein（ AFP）,with the differences statistically significant（ r =-0. 626 8,r = 0. 536 7,P〈 0. 05）. Moreover,the increased levels of GPC3 mRNA were related with patients with hepatitis B virus infection（ P〈 0. 05）. Conclusion ZHX2 mRNA was significantly correlated with GPC3 mRNA,ZHX2 might remote the proliferation of hepatocellular carcinoma through increasing the level of GPC3 mRNA.