大别山五针松内生放线菌WP-1活性代谢产物制霉色基素发酵条件优化研究

Optimization of Fermentation Conditions of Actinomycetes Strain WP-1 from Pinus Dabeshanensis for Fungichromin Production

目的通过优化内生放线菌WP-1的发酵条件,提高制霉色基素(funguchromin,FC)的产量。方法采用单因素试验和正交试验分析不同速效、迟效碳源,不同速效、迟效氮源以及不同无机盐对FC产量的影响,优化放线菌WP-1的培养基组分;采用单因素试验分析不同的培养基初始pH值、培养温度、接种量、装液量、摇床转速对FC产量的影响,优化放线菌WP-1的培养条件。结果优化后的发酵条件为葡萄糖1%,可溶性淀粉4%,蛋白胨0.5%,花生饼粉9%,MnSO_40.000 5%,ZnSO_4 0.000 5%,CuSO_4 0.000 2%;初始pH 5.5,发酵温度28℃,装液量50 m L培养基/250 m L三角瓶,接种量8%,转速180 r·min^(-1)。在此条件下,FC的产量可达159.0 mg·L^(-1),与初始发酵条件相比,提高了4.9倍。结论该方法优化了WP-1产生FC的发酵条件,为下一步规模发酵提供了基础数据。

OBJECTIVE To increase the yield of fungichromin（FC） in actinomycetes strain WP-1 through optimizing fermentation conditions. METHODS The fermentation medium components were optimized by single factor and orthogonal experiments to investigate the effect of different available or slow-acting carbon sources, nitrogen sources and different inorganic salts on the yield of FC. The culture conditions of actinomycetes strain WP-1 were optimized by single factor experiments to investigate the effect of different initial p H values of medium, culture temperatures, inoculation amount, fermentation volume and rotational speeds on the yield of FC. RESULTS The optimization results were as follows： glucose 1%, soluble starch 4%, peptone 0.5%, peanut powdery cake 9%, MnSO_4 0.000 5%, ZnSO_4 0.000 5%, CuSO_4 0.000 2%; initial pH 5.5, T 28 ℃, loaded liquid 50 m L medium/250 m L triangular flask, inoculum size 8%, rotate speed 180 r min^（-1）. Under the optimized fermentation conditions, FC yield of actinomycetes strain WP-1 were 159.0 mg·L^（-1）, about 4.9 times of that under the initial fermentation conditions. CONCLUSION The best fermentation condition to produce FC is obtained so that this paved the way for next step of large scale fermentation.