鸡胚绒毛膜尿囊膜血管试验法用于评价化妆品眼刺激性的可行性研究

Research on the Feasibility of Using Chorioallantoic Membrane Vascular Assay to Evaluate Eye Irritation of Cosmetics

目的研究鸡胚绒毛膜尿囊膜血管试验法(chorioallantoic membrane vascular assay,CAMVA)评价化妆品眼刺激性的可行性,并用改良后的方法对14种市售化妆品进行眼刺激性的评价。方法通过控制样品的浓度、与鸡胚绒毛膜尿囊膜的接触时间,以及对评价体系的改良,尝试建立一套标准的CAMVA法评价化妆品眼刺激性的方法。结果护肤类产品无刺激性,用后冲洗类、染发类产品的浓度、与鸡胚绒毛膜尿囊膜的接触时间均与刺激性大小成正比。在3%的浓度下,用后冲洗类产品的最佳接触时间为20 min,体内外试验结果一致性为90%(9/10);染发类产品为5 min,体内外试验结果一致性为100%(2/2)。结合改良后的评价体系可做到刺激性分级,体内外试验结果一致性为用后冲洗类产品80%(8/10),染发类产品50%(1/2)。结论用CAMVA法可以对化妆品的眼刺激性进行评价,但实验条件和判定标准还需进一步优化。

OBJECTIVE Study on the feasibility of using Chorioallantoic Membrane Vascular Assay to evaluate eye irritation of cosmetics, and evaluating 14 kinds of cosmetics by improved method. METHODS Establish a standard method on eye irritation using CAMVA of cosmetics by controlling exposure time and sample concentration, and improving the evaluation system. RESULTS Skin care products are non-irritating. There was a positive correlation between exposure time, sample concentration and the stimulation intensity of hair dyes and after using rinse-off cosmetics. At 3% concentration, the best exposure time is 20 min for after using rinse-off cosmetics, the classification consistency of CAMVA and Draize test was 90%（9/10）, and 5min for hair dyes, the classification consistency of CAMVA and Draize test was 100%（2/2）. Irritation can be graded by using the improved evaluation system, the classification consistency of CAMVA and Draize test of after using rinse-off cosmetics was 80%（8/10）, and the classification consistency of CAMVA and Draize test of hair dyes was 50%（1/2）. CONCLUSION CAMVA method can be applied to predict eye irritation of cosmetics,but the experimental conditions and evaluation system need to be further refined.