HPLC法同时测定跌打丸中3种有效成分的含量

Content Determination of Three Effective Components in Dieda Pills by HPLC

目的探讨建立同时测定跌打丸中芍药苷、川续断皂苷Ⅵ和丹皮酚含量的方法。方法采用Agilent ZORBAX C_(18)色谱柱(4.6 mm×250 mm,5μm);流速为0.8 m L·min^(-1),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,进行梯度洗脱;检测波长为230,212,274 nm。结果芍药苷进样量在0.514 8~1.544 5μg与峰面积线性关系良好(r=0.999 7),平均回收率为97.01%(RSD=0.94%,n=6)。川续断皂苷Ⅵ在3.023 8~9.071 4μg线性关系良好(r=0.999 2),平均回收率为98.44%(RSD=0.26%,n=6)。丹皮酚在0.101 2~0.303 6μg线性关系良好(r=0.999 8),平均回收率为97.84%(RSD=0.42%,n=6)。结论建立的方法简便可行,重复性好,灵敏度高,可有效控制跌打丸的质量。

OBJECTIVE To establish the HPLC method for content determination of paeoniflorin, Asperosaponin Ⅵand paeonol in Dieda pills. METHODS The contents of paeoniflorin, Asperosaponin and paeonol was determined by an HPLC Ⅵsystem using following conditions： the column was Agilent ZORBAX C_（18）（4.6 mm×250 mm, 5 μm） with a mobile phase of acetonitrile（A）-0.1% phosphoric acid（B） in gradient elution mode at the flow rate of 0.8 m L·min^（-1）, the detection wavelength was at 230, 212 and 274 nm. RESULTS The linear range of paeoniflorin was 0.514 8-1.544 5 μg（r=0.999 7） and its average recovery rates（n=6）were 97.01%（RSD=0.94%）. The linear range of Asperosaponin Ⅵwas 3.023 8-9.071 4 μg（r=0.999 2） and its average recovery rates（n=6）were 98.44%（RSD=0.26%）. The linear range of paeonol was 0.101 2-0.303 6 μg（r=0.999 8） and its average recovery rates（n=6）were 97.84%（RSD=0.42%）. CONCLUSION The HPLC method is simple, accurate, highly selective and reproducible, thus it can be used as quality control of Dieda pills.