电针对糖尿病胃轻瘫模型大鼠胃电及胃窦ghrelin的影响(英文)

Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis

目的:观察电针对糖尿病胃轻瘫(DGP)模型大鼠胃电及胃窦ghrelin的影响。方法:将50只Sprague-Dawley(SD)大鼠随机分为A组、B组、C组、D组和E组,每组10只,A组为空白对照组,不接受任何干预;B组、C组、D组和E组采用单次腹腔注射2%的链脲佐菌素(STZ)并配合8星期高糖高脂饮食建立DGP大鼠模型。B组为模型组,不接受治疗;C组为电针穴位组,采用电针足三里、梁门和三阴交治疗;D组为电针非穴位组,采用电针足三里、梁门及三阴交的对照点治疗;胃复安对照组大鼠接受1.7%胃复安药液[10 mL/(kg·bw)]灌胃。用血糖仪检测大鼠血糖值;以酚红为标记物,检测大鼠胃排空率,BL-420F生物机能实验系统检测胃电,酶联免疫吸附测定法(ELISA)检测胃窦ghrelin含量,实时荧光定量聚合酶链反应(RT-PCR)检测ghrelin mRNA的表达。结果:与A组比较,治疗前和治疗后B、C、D和E组的血糖均显著增高(均P<0.01);治疗后B组大鼠胃排空率显著降低(P<0.01),治疗后B、C、D和E组小肠推进率显著降低(均P<0.01),治疗后C组胃窦ghrelin含量显著降低(P<0.01),治疗后B、C、D和E组胃窦ghrelin mRNA表达显著升高(均P<0.01),治疗后B组和D组胃电平均振幅显著降低(均P<0.01);与B组比较,C组治疗后血糖明显下降(P<0.05),治疗后C、E组胃排空率及小肠推进率明显升高(P<0.05,P<0.01),D组小肠推进率明显升高(P<0.05),治疗后C组胃窦ghrelin含量及mRNA表达显著降低(P<0.01),E组ghrelin mRNA表达显著降低(P<0.01),治疗后C组胃电平均振幅明显增加(P<0.05);与D组比较,治疗后C组胃窦ghrelin含量及mRNA表达显著降低(P<0.01);与E组比较,治疗后C组胃窦ghrelin含量显著降低(P<0.01)。结论:电针足三里、梁门及三阴交可以调节DGP模型大鼠的血糖水平,增强胃电活动,促进胃排空,调节胃窦ghrelin水平及下调ghrelin mRNA的表达。

Objective： To observe the effect of electroacupuncture（EA） on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis（DGP）.Methods： Fifty Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E,10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin（STZ）, combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）. Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/（kg·bw）] by gavage. Rat＇s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420 F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay（ELISA）; the expression of ghrelin mRNA was detected by real-time polymerase chain reaction（RT-PCR）.Results： Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment（all P〈0.01）; after treatment, the gastric emptying rate of group B was significantly decreased（P〈0.01）,the migration rates of small intestine in group B, C, D and E were significantly lower（all P〈0.01）, and the protein content of ghrelin in group C was significantly decreased（P〈0.01）; the expressions of ghrelin mRNA were significantly increased in group B, C, D and E（all P〈0.01）, the mean amplitudes of electrogastrogram in group B and D were significantly decreased（both P〈0.01）. After treatment, compared with group B, the blood glucose of group C was significantly decreased（P〈0.05）, the gastric emptying rate and small intestine migration rate were significantly increased in group C and E（P〈0.05, P〈0.01）, the small intestinal migration rate was significantly increased in group D（P〈0.05）, the expression of ghrelin in protein and mRNA in group C was significantly lower（P〈0.01）, the expression of ghrelin mRNA in group E was significantly lower（P〈0.05）, and the mean amplitude of electrogastrogram in group C was significantly increased（P〈0.05）. After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased（P〈0.01）. After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased（P〈0.01）.Conclusion： EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6） could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.