资生肾气丸对痛风性关节炎大鼠IL-1表达水平的影响

Effect of Zisheng Shenqi Decoction on Expression of IL-1β and Caspase-1 in Gouty Arthritis Rats

目的:观察资生肾气丸对痛风性关节炎大鼠血清中白介素1β(IL-1β)及大鼠滑膜半胱氨酸天冬氨酸特异蛋白酶-1(Caspase-1)的表达水平的影响,探讨资生肾气丸防治痛风性关节炎(GA)的作用机制。方法:将84只SD大鼠随机分为7组:空白组、模型组、中药痛风舒对照组、西药秋水仙碱对照组及资生肾气丸复方低、中、高剂量组,每组12只。采用尿酸钠联合氧嗪酸钾诱导大鼠痛风性关节炎模型,应用ELISA方法检测各组大鼠血清中IL-1β含量,应用免疫组化方法检测大鼠滑膜Caspase-1含量。结果:模型组和空白组IL-1β、Caspase-1含量差异有统计学意义(P<0.01);西药秋水仙碱对照组、资生肾气丸复方低、中、高剂量组IL-1β和Caspase-1含量与模型组比较差异有统计学意义(P<0.01);中药中剂量组与痛风舒对照组比较,IL-1β、Caspase-1含量差异有统计学意义(P<0.01),但与西药对照组比较差异无统计学意义(P>0.01)。结论:资生肾气丸可影响痛风性关节炎大鼠IL-1β和Caspase-1表达,对痛风性关节炎具有防治作用。

Objective： To observe the effect of Zisheng Shenqi Decoction on expression of IL-1β and Caspase-1 in gouty arthritis rats and to explore the mechanism of Zisheng Shenqi Decoction in the treatment of GA. Methods： Eighty four rats were randomly divided into seven groups： blank control group,model group,herbal control group,western medicine control group,high dosage herbal group,medium dosage herbal group and low dosage herbal group,twelve rats in each group. The uric acid sodium and oxygen polybenzoxazines potassium were used to induce rat gouty arthritis model. ELISA was used to detect the concentration of serum IL-1β in rats and analyze the data. Using immunohistochemical method to detect the contents of synovial Caspase-1 in rats. Results： The differences of IL-1β and Caspase-1 level between model group and blank group had statistical significance（ P 0. 01）. The expression of IL-1β and Caspase-1 in model group was also apparently different from that in western medicine control group,highdosage herbal group,medium dosage herbal group and low dosage herbal group,the differences had statistical significance（ P 0. 01）; The expression of IL-1β and Caspase-1 in medium dosage herbal group was apparently different from that in herbal control group（ P 0. 01）,but had not apparently difference from western medicine control group（ P 0. 01）. Conclusion： Zisheng Shenqi Decoction can inhibit the expression of IL-1β and Caspase-1 in gouty arthritis rats. It has certain curative effect gouty arthritis.