水体细菌微生物多波长透射光谱解析模型

An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water

细菌多波长透射光谱包含有细菌结构、组分、浓度等信息,这些特征信息的有效提取是实现细菌微生物快速识别与检测的基础。以水体常见的大肠埃希氏菌(大肠杆菌)为研究对象,采用紫外-可见分光光度法获得了其多波长透射光谱;基于Mie散射理论,在充分考虑水体大肠杆菌散射和吸收特性的基础上,构建了240~900nm波段范围内细菌微生物多波长透射光谱的解析模型;基于该模型对250~750nm特征波段范围内的光谱进行解析,获得了大肠杆菌的体积、粒径、结构及浓度等相关参数,并将这些参数与文献及实验得到的结果进行了对比验证。结果表明,建立的多波长透射光谱解析模型能够准确表征水体细菌微生物的光谱特征,该模型可为水体细菌微生物的快速识别分析和检测提供关键数据。

Multi-wavelength transmittance spectra of bacteria in water contain lots of information on their characteristics such as structure,constituent and concentration,and the effective extraction of the information is the foundation of rapid identification and detection of bacteria.The transmittance spectrum of Escherichia coli is recorded with a ultraviolet-visible spectrophotometer.According to Mie scattering theory and absorption and scattering properties,bacterial multi-wavelength transmittance spectrum analytical model in 240-900 nm band is constructed.Based on the model,the spectrum in the range of 250-750 nm is analyzed,the related parameters such as volume,diameter,structure and concentration of Escherichia coli cells are obtained,and these parameters are then compared with the literature data or experimental data.It is shown that the proposed multi-wavelength transmission spectrum model can accurately characterize the spectral features of bacteria in water,and the model can provide important parameters for the rapid analysis and detection of bacteria in water.