柘木提取物抗胃肠道肿瘤的免疫机制研究

Immunologic Mechanism of Inhibition on Gastrointestinal Tract Tumors of ZHEMU Extract

目的观测柘木提取物对正常或荷瘤动物免疫功能变化的影响,探讨其抗癌作用机制。方法给药后观察正常小鼠碳粒廓清能力和腹腔巨噬细胞吞噬功能,检测荷瘤小鼠淋巴细胞转化和自然杀伤(NK)细胞毒活性。结果柘木提取物对正常小鼠碳粒廓清能力和腹腔巨噬细胞吞噬功能、对荷C26肠癌小鼠淋巴细胞转化和NK细胞活性均有增强作用,最低有效剂量均为0.3g/kg,相当于柘木生药5.46g/kg,为临床单日使用剂量的1.3倍。结论柘木提取物对胃肠道肿瘤抑制作用机制与其对胃肠道肿瘤细胞的直接抑制作用以及提高机体特异性和非特异性免疫功能有关。

Objective To observation the changes of immune function in normal or tumor bearing animal after dose ZHEMU extract. Methods The carbon particle clearance test, macrophage phagocytosis test. NK cells activity and lymphocyte transformation in tumor bear animal are detected. Results The carbon granula clearance ability and phagocytosis of macrophage cell in abdominal on normal mice, and the lymphocyte transformation and natural killer （NK） cell activity in tumor bearing mice were enhanced after dosing, and the lowest effective dose were 0.3 g/kg, equivalent to the crude herb 5.46 g/kg, which was 1.3 times of clinical daily dose. Conclusions ZHEMU extract can restrain the growth of gastrointestinal tract tumors, and its mechanism may be related to the direct inhibition of the gastrointestinal tract cancerous cells growth and the improvement of the body＇s specific and non-specific immune function.