肉用绵羊超数排卵效果实例分析

A Retrospective Survey Analysis of Superovulation in Mutton Sheep

【目的】超排胚胎移植技术是提高绵羊遗传潜力的一项重要应用技术,但影响供体超数排卵的因素很多,也需要大量数据支持。【方法】以影响供体超排的因素做回顾性调查分析,从激素来源、肉羊品种、日粮水平、年龄、超排间隔和超排剂量等6个方面观察对供体超排的影响。【结果】国产FSH超排效果与进口FSH差异不显著(P>0.05),肉羊品种间无显著差异性(P>0.05),年龄对供体超排反应影响显著(P<0.05),营养对供体的超排反应显著(P<0.05),供体以短间隔超排重复利用获得胚胎数量最多,增加超排剂量能显著改善超排反应(P<0.05),但对重复利用供体差异不明显(P>0.05)。【结论】对超排反应有影响的因素分为显著的和不显著的因素,显著的因素包括年龄、营养、超排间隔、超排剂量,而不显著的因素为品种。

[Objective] Multiple ovulation and embryo transfer （ MOET ） is an important applied tech-nology to improve the genetic potential of sheep. There are many factors that affect donor superovulation, and a large amount of data is needed to support it.[Method] A retrospective survey about the factors such as Folli-cle stimulating hormone resource, breed, daily ration, age, superovulation interval and dosage were analyzed and summarized between superovulation responses in our application for mutton sheep.[Result] The results showed that domestic FSH was equal to Canadian FSH in superovulation response （P 〉0. 05 ） , different breeds of mutton sheep had no significant difference in superovulation response （ P 〉 0. 05 ） ; It was remarkable between youth and adult in the superovulation response （ P 〈 0. 05 ） ; It was the same to ration supply （ P 〈 0. 05） . The donor received the largest number of embryos when repeatedly superovulated by short internal, the increased FSH dosage impacted superovulation response signivicantly in mutton sheep （P〈 0.05 ） , but continuous repeatedly superovulation had no obvious effect （P〉0.05 ） .[Conclusion] In conclusion, the in-fluencing factors could be divided into remarkable and indistinctive factors. Age, daily ration, superovulation interval and dosage can be relegated to the remarkable factors and breed can be classified to the indistinctive factors.