C13呼气试验及荧光定量PCR法在体检中心幽门螺杆菌检测中的应用研究

Application value of C13 breath test and fluorescence quantitative PCR on detection of helicobacter pylori in physical examination center

目的:观察和比较分析C13呼气试验及荧光定量PCR法在体检中心幽门螺杆菌检测中的应用效果。方法:选取2014-11~2015-01在我院行胃镜检查患者76例,取其胃黏膜组织分别用荧光定量PCR法及C13呼气试验检测幽门螺杆菌。结果:C13呼气试验和荧光定量PCR法检测幽门螺杆菌阳性率分别为64.47%(49/76)和65.79%(50/76),阴性率分别为35.53%和34.21%,差异无统计学意义(P>0.05),两种检测方法具有较好的相关性(P<0.05)。结论:C13呼气试验和荧光定量PCR法检测幽门螺杆菌具有相似的阳性率和阴性率,且具有较好的相关性。但C13呼气试验具有费用低、无创且检测时间短的优点,更值得临床推广。

Objective.. To observe and compare application value of C13 breath test and fluorescence quantitative PCR on detection of helicobacter pylori in the physical examination center. Methods. Seventysix patients were choosen with gastroscopy examination from November 2014 to January 2015, and were taken gastric mucosal tissue for fluorescence quantitative PCR and C13 breath test on detection of helicobacter pylori.Results.C13 breath test and fluorescence quantitative PCR detection of helicobacter pylori positive rate were 64.47% （49/76） and 65.79% （50/76）, negative rate were 35.53% and 34.21% respectively, the difference had no statistical significance （P〉0.05）.Two methods had a good correlation （P〈 0.05）.Conclusion. C13 breath test and fluorescence quantitative PCR detection of helicobacter pylori has a similar positive rate and negative rate,and has good correlation.But the C13 breath test has low cost, noninvasive, and the advantage of short detection time, more worthy of clinical promotion.