艾塞那肽改善非酒精性脂肪性肝病大鼠抗氧化能力的研究

Exenatide improving antioxidant ability of nonalcoholic fatty liver disease of rats

目的 探讨艾塞那肽改善非酒精性脂肪性肝病（NAFLD）大鼠的抗氧化能力.方法 高脂饮食12周成功建立大鼠NAFLD模型,将120只大鼠随机分为空白对照组（CON组）,模型对照组（HFD组）,艾塞那肽低、中、高剂量治疗组（ELD组、EMD组、EHD组）以及多烯磷脂酰胆碱治疗组（PDC组）,每组20只.治疗组均给予艾塞那肽4、8周后,分别处死大鼠各半,肝脏切片HE染色,测定肝脏和血清抗氧化指标.结果 艾塞那肽治疗的剂量越大,时间越长,NAFLD大鼠肝脏脂肪浸润和炎症反应明显减轻,脂肪颗粒明显减少;血清和肝脏总抗氧化能力明显改善.结论 艾塞那肽可以明显改善NAFLD大鼠的抗氧化能力,治疗NAFLD.

Objective To investigate the exenatide effect on fat-induced nonalcoholic fatty liver disease（NAFLD） of rat.Methods NAFLD models were established after 12 weeks of high fat diet feeding,a total of 120 SD rats were randomly divided into control group（CON group）,model group（HFD group）,exenatide low,medium,high dose group（ELD group,EMD group,EHD group） and polyene phosphatidylcholine treatment group（PDC group）,with 20 rats in each group.After given appropriate treatment,half of the rats were sacrificed after 4 and 8 weeks.HE staining of liver slices,antioxidant factors were performed.Results With the dose of exenatide became larger,and the time got longer,the liver fat and the inflammatory response were significantly reduced and the fat particles were significantly reduced;serum and liver total antioxidant capacity was significantly improved.Conclusion Exenatide improves antioxidant ability of NAFLD of rats.