光激活定位超分辨荧光成像的罗丹明分子设计

Molecular design of rhodamine dyes for photoactivated localization microscopy

光激活定位显微技术(PALM)等超分辨成像技术的发展为高水平生命科学研究提供了突破光学衍射极限的研究工具.实现超分辨荧光成像的核心要素之一是高性能荧光染料,而罗丹明染料由于其优良的光学性质已成为设计PALM染料的热门选择.本文通过对PALM超分辨成像特点分析,给出PALM罗丹明染料的设计要求,并对已报道的PALM超分辨罗丹明染料的设计策略及其优缺点进行比较分析,希望通过对分子设计策略的综述,为研究者设计PALM超分辨罗丹明染料提供了一些帮助.

Super resolution imaging including photactivated localization microscopy has provided diffraction breaking tool for biological research. One of the keynotes of super resolution imaging is high performance dye. Resulting from its high fluorescent performance, rhodamine has been widely chosen as the fluorophore core for designing PALM dye.This paper demonstrates the qualification of PALM dye for super resolution imaging. Subsequently through analyzing the gap between rhodamine designs, both the strengths and weaknesses of strategies are illustrated. This mini review on molecular design of rhodamine may help the future development of high performance PALM dye.