自身免疫性肝病患者相关自身抗体检测探析

Study on Test of Related Autoantibodies in Patients with Autoimmune Liver Disease

目的分析自身免疫性肝病(AILD)患者相关自身抗体检测免疫学指标及临床意义。方法整群选取该院2015年9月—2016年9月收治的436例肝功能反复异常患者临床资料作为研究对象,其中AILD患者为160例,病毒性肝炎患者为276例。使用间接免疫荧光法(IIF)对两组患者进行抗核抗体(ANA)、抗线粒体抗体(AMA)、抗中性粒细胞胞浆抗体(ANCA)、抗平滑肌抗体(SMA)检测。结果 AILD患者ANA、AMA、ANCA、SMA、抗MPO抗体阳性率分别是73例、69例、77例、47例、62例,所占比分别为45.63%、43.13%、48.13%、29.38%、38.75%,与病毒性肝炎患者进行对比差异有统计学意义(P<0.05)。对于两组患者自身抗体的检测结果进行比较AID患者与病毒性肝炎患者在AILD在ANCA及p-ANCA检出率方面差异有统计学意义(P<0.05)。结论 AILD患者相关自身抗体联合检测,能够有效地应用于AILD的检出、诊断及临床分型上,有利于区别AILD与病毒性肝炎的诊断,值得临床大力推广。

Objective To analyze the immune indexes and clinical significance of test of related autoantibodies in patients with autoimmune liver disease. Methods Group selection 436 cases of patients with repeated abnormalities of liver function in our hospital from September 2015 to September 2016 were selected including 160 cases of AILD patients and 276 cases of patients with viral hepatitis, and the ANA, AMA, ANCA and SMA were tested by the IIF. Results The positive rates of ANA, AMA, ANCA, SMA and anti-MPO antibody in AILD patients were respectively 73 cases, 69 cases, 77 cases, 47 cases, 62 cases, accounting for 45.63%, 43.13%, 48.13%, 29.38%, 38.75%, and there were obvious differences between the AILD patients and viral hepatitis patients(P<0.05), and there were obvious differences in the test rates of AILD, ANCA and p-ANCA between the two groups(P<0.05). Conclusion The autoantibodies combined test of AILD patients can be effectively applied in the test, diagnosis and clinical classification of AILD, which is conducive to distinguishing the diagnosis of AILD and viral hepatitis, and it is worth great promotion in clinic.