TBB对乳腺癌细胞miR-411表达及细胞凋亡的影响

Expression of miR-411 in breast cancer cells with TBB treatment and its relation to cell apoptosis

目的观察TBB作用下乳腺癌细胞中miR-411的表达,研究过表达miR-411对乳腺癌细胞凋亡和细胞周期改变的影响。方法 Real-time PCR验证生物芯片检测的差异miRNAs,流式细胞术分析转染前后细胞周期及细胞凋亡情况,针对miR-411进行下游靶基因预测,以real-time PCR验证预测结果。结果 TBB作用下MCF-7细胞株中miR-411显著升高;与对照组相比,转染组早期凋亡细胞比例升高;Fox O1 mRNA表达含量显著增高。结论TBB能够改变乳腺癌M CF-7细胞株中miR-411的表达,过表达miR-411能够抑制乳腺癌细胞株的凋亡,其机制可能与通过影响Fox O1表达有关。

Objective To investigate the expression o f miR-411 in breast cancer cells after treated with TBB and its relations to cell cycle and apoptosis. Methods Breast cancer MCF-7 c e l ls were transfected with hsa-miR-411-mimic and treated by 4,5,6,7- tetra-bromo-benzotriazole （TBB）. The expression of microRNAs in MCF-7 cel ls were detected by microRNAs array, and real-time PCR. The cel l cycle and apoptosis of MCF-7 cel ls were analyzed by flow cytometry after hsa-miR-411 mimics transfection. The possible target of miR-411 was forecasted by bioinformatics tools and the expression of the possible target gene was detected by real-time PCR. Results Real-time PCR showed that the expression of miR-411 was changed after TBB treatment in MCF-7 cel ls. Flow cytometry showed that early apoptotic cel ls in transfected groups dramatically increased compared to control groups. Real-time PCR showed that the expression of FoxOl mRNA was significantly upregulated. Conclusion The expression of miR-411 is altered after the introduction of CK2 inhibitor TBB. Transfection with miR-411 mimics can increase phase G0/G1 ce l l s , inhibiting cell proliferation and promoting apoptosis. FoxO1 gene may be involved in the pathway of tumor cell proliferation and apoptosis.