急性缺血性卒中早期血浆miRNAs水平与房颤相关性研究

Research on Correlation between the Early Plasma of Acute Ischemic Stroke miRNAs Level and Atrial Fibrillation

目的探讨和分析急性缺血性卒中早期血浆miRNAs水平和房颤发生之间的相互关系。方法整群选取该院神经内科在2016年10月—2017年2月期间收治的急性缺血性卒中患者195例为分析对象,依据其是否合并有房颤又划成持续性房颤组(65例)和阵发性房颤组(65例)以及非房颤组(65例),并以同期在该院进行体检的健康体检者65名作为健康组,用2-ΔΔCt法来对miRNAs具有的相对表达量实施测试。结果持续性房颤组患者的miR-328为(5.51±0.76),显著高于阵发性房颤组;持续性房颤组的miR-150为(0.63±0.52),显著低于健康对照组;健康对照组和非房颤组的miR-29b分别为(1.00±0.39)、(0.91±0.27),均明显高于阵发性房颤组;以上组间对比差异有统计学意义(P<0.05);持续性、阵发性房颤组的miR-29b分别为(0.50±0.39)、(0.49±0.41),该两组的miR-29b对比差异无统计学意义(P>0.05)。结论早期血浆miRNAs水平中的miR-328、miR-29b和miR-150指标与急性缺血性卒中合并房颤的发生呈显著相关。因此,这些分子可能有望作为急性缺血性卒中患者是否合并房颤的有效预测指标。在预测急性缺血性卒中患者是否具有阵发性房颤时,有望将miR-29b的实际水平作为有效的参考依据,应引起足够的重视。

Objective To study and analyze the correlation between the early plasma of acute ischemic stroke miRNAs level and atrial fibrillation. Methods 195 cases of patients with acute ischemic stroke admitted and treated in our hospital from October 2016 to February 2017 were cluster selected and divided into two groups with 65 cases in each according to whether the patients were with atrial fibrillation or not, and 65 cases of healthy people were selected as the healthy group at the same period, and the relative expression amount of the miRNA was tested by the 2-ΔΔCt method. Results The miR-328 was in the continuous group was obviously higher than that in the paroxysmal group, which was(5.51±0.76)in the continuous group, and the miR-150 was(0.63 ±0.52) in the continuous group, which was obviously lower than that in the healthy control group, and the miR-29 b in the healthy control group and non-atrial fibrillation group was respectively(1.00±0.39) and(0.91±0.27), which were obviously higher than those in the paroxysmal group, and the differences between groups were obvious with statistical significance(P<0.05), and the miR-29 b in the continuous and paroxysmal groups were respectively(0.50±0.39) and(0.49±0.41), and the difference was obvious with statistical significance(P>0.05). Conclusion The miR-328, miR-29 b and miR-150 in the early plasma miRNAs level have an obvious correlation with the occurrence of acute ischemic stroke complicated with atrial fibrillation, therefore, which can be expected to be effective predictive indexes of whether the acute ischemic stroke patients are with atrial fibrillation or not, and the practical level of miR-29 b can be used as an effective reference basis of prediction, and we should attach enough importance.