抗菌肽在大肠杆菌中的融合表达策略

Fusion Expression Strategy of Antimicrobial Peptides in Escherichia Coli

抗菌肽抗菌谱广、活性稳定,具有与抗生素不同的抗菌机制,在抑杀病原微生物的同时不易产生耐药性,在免疫调节和抗感染方面具有巨大的发展潜力,能够发展为取代抗生素的新型药剂,在食品、饲料、畜禽养殖等领域也具有重要的应用价值。基因工程技术是降低抗菌肽生产成本的主要方式,其中融合表达在提高抗菌肽产量方面起到了重要作用。综述了抗菌肽在大肠杆菌中融合表达的国内外研究进展,探讨了抗菌肽在大肠杆菌中融合表达的策略,并对高通量融合表达抗菌肽提出了建议。

Antimicrobial peptides have a wide antimicrobial spectrum and stable activity, with different antibacterial mechanisms from antibiotics, they result in a higher efficacy against pathogens. At the same time, they＇re difficult to form drug resistance. Antimicrobial peptides have great development potential in immune regulation and anti-infection, so they＇re able to develop new agents, to replace antibiotics. Besides, they have immense applied value in food, forage, poultry breeding and so on. Genetic engineering is the main way to reduce the production costs in antimicrobial peptides, furthermore, the fusion expression plays a important role in improving production. In this paper, we summarized the research progress of antimicrobial peptides＇ fusion expression in E. coli, at home and abroad. Also, the fusion strategy in E. coli are discussed, and we proposed our own view on the high flux fusion expression of antimicrobial peptides.