人参叶中多种农药残留的气相色谱-串联质谱快速分析方法研究

Studies on the Determination Method of Pesticide Multi-residues in Ginseng Leaves by Gas Chromatography-Mass Spectrometry

目的:应用气相色谱-质谱技术建立一种快速检测人参叶中74种农药残留的分析方法。方法:样品用乙腈提取,经QuEChERS试剂盒净化后,利用气相色谱-质谱在分时段多反应监测模式下进行定性分析,采用内标法定量。结果:农药对照品在0.05~0.4mg·kg^(-1)范围内线性关系良好,回收率在84%~112%之间,相对标准偏差(RSD)<15%(n=6)。结论:该方法快速且具有良好的准确度和灵敏度,适用于人参叶中多种农药残留的测定。

Objective： To develop an analysis method based on gas chromatography- mass spectrometry （GC-M$） to determine 74 kinds of pesticide residue for ginseng leaves rapidly. Methods：The sample was extracted with acetone, purified by QuEChERS kit. Qualitative analysis was carried out by GC-MS under multiple reaction monitoring mode （MRM）. Internal standard method was used for quantitative analysis. Results： The linear range of this method was 0.05 ~ 0.4 mg · kg1. The recovery was 84 % ~ 112 % with relative standard deviations （RSD） better than 15 % （n= 6）. Conclusion： The method is rapid, accurate and sensitive. It is suitable for the multiresidues analysis of pesticides in ginseng leaves.