大麻素受体2与子宫颈癌组织内浸润T细胞亚群的关系

The relationship between cannabinoid receptor 2 and infiltrating T-cell subsets in cervical cancer tissue

目的研究大麻素受体2(cannabinoid receptor 2,CB 2)在人子宫颈癌组织中的表达及其与肿瘤内浸润T细胞亚群,包括FOXP 3+T、CD 4+T、CD 8+T细胞的关系。方法选取2016年德州市中医院病理科保存的人体手术切除标本90例,纳入30例正常人宫颈组织为对照A组、30例人高级别宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)Ⅱ~Ⅲ级组织为对照B组、30例人子宫颈癌组织为观察组。采用免疫组织化学法及实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,RT-PCR)检测CB 2在各组中表达情况,采用间接免疫荧光双标法检测观察组组织内浸润FOXP 3+、CD 4+T、CD 8+T细胞数目。结果 CB 2蛋白及mRNA在3组中均有表达,且在观察组中表达最高、对照B组中次之、对照A组中表达量最低,各组间差异均有统计学意义(P<0.05);观察组CB 2蛋白及mRNA的表达均与组织内浸润的CD8+T呈负相关(P<0.05)。结论 CB 2在人子宫颈癌组织内存在高表达现象,且与组织内浸润CD 8+T细胞数量减少有一定相关性。

Objective To study the expression of cannabinoid receptor 2 （ CB 2 ） in human cervical cancer tissues and the relationship between CB 2 and infiltrating T - cell subsets, inclueding FOXP 3 ＋ T, CD 4 ＋ T and CD 8 ＋ T cells. Methods 30 normal cervical tissues were involved in control group A, 30 high grade cervical intraepithelial neoplasia （ CIN Ⅱ - Ⅲ ） tissues were involved in control group B, and 30 cervical cancer tissues were involved in observation group. Immunohistochemistry and quantitative real -time polymerase chain reaction（ RT- PCR） were used to detect the expression of CB 2 in each group. FOXP 3 ＋ T, CD 4 ＋ T and CD 8 ＋ T cells in the observation group were detected by double immunofluorescence labeling method. Results CB2 protein and mRNA expressed in all tissues. The expression was highest in the observation group, followed by control group B and control group A. The differences between groups were statistically significant（ P 〈 0. 05 ）. The expression of CB 2 protein and mRNA in the observation group were negative correlated with CD 8 ＋ T cells （ P 〈 0. 05 ）. Conclusion CB 2 is highly expressed in the cervical cancer tissue, and there is a certain correlation with the decrease of the number of infiltrating CD 8 ＋ T cells in the tissue.