儿童系统性红斑狼疮患者外周血淋巴细胞表面CD95的表达及临床意义

Expression and significance of CD95 in peripheral blood lymphocytes of children with new-onset systemic lupus erythematosus

目的:探讨儿童系统性红斑狼疮(systemic lupus erythematosus,SLE)外周血淋巴细胞表达CD95的特征及与疾病活动性和其他免疫学指标间的关系。方法:使用流式细胞术检测60例SLE患儿和20例对照外周血T淋巴细胞亚群和B淋巴细胞表面CD95的表达,并分析其与SLE疾病活动性以及实验室检查之间的关系。结果:初发SLE患儿外周血中CD4+T细胞表面CD95的表达显著高于对照组,差异有统计学意义(P<0.05);初发SLE患儿外周血中CD19+B细胞表面CD95的表达显著高于健康儿童(P<0.05);CD19+CD95+B细胞的比例和SLE疾病活动性呈正相关(r=0.4,P<0.05);CD4+CD95+T细胞的比例和SLE疾病活动性呈正相关(r=0.3,P<0.05),CD4+CD95+T细胞的比例和外周血抗双链DNA抗体(anti-ds DNA Abs)的水平呈正相关(r=0.2,P<0.05);治疗后SLE患儿外周血中CD19+CD95+B细胞和CD4+CD95+T细胞的比例均有显著下降,差异有统计学意义(P<0.05)。结论:儿童SLE患者外周血中淋巴细胞表达CD95的水平显著升高,且与SLE的疾病活动性及血清中抗双链DNA抗体相关,可以作为SLE的评价指标。

Objective： To investigate the expression of CD95 in peripheral blood lymphocytes of children with systemic lupus erythematosus （SLE） and its relationship with disease activity and other immunological parameters. Methods： Flow cytometry was used to detect the expression of CD95 on T and B lymphocytes in peripheral blood of 60 children with SLE and 20 healthy children, and analyze the relationship between the expression of CD95 and disease activity. Results： The expression of CD95 in CD4＋ T cells in peripheral blood of children with SLE was significantly higher than that of healthy children, the expression of CD95 in CD 19＋ B in peripheral blood of children with SLE was significantly higher than that of healthy children. The proportion of CD4＋ CD95＋ Tcells was positively correlated with SLE disease activity,The proportion of CD 19＋ CD95＋ B cells was positively correlated with SLE disease activity, qlae proportion ofCD4＋ CD95＋ T cells was positively correlated with the level of anti-double stranded DNA antibody （anti-dsDNA Abs） in peripheral blood. Conclusion： The expression level of CD95 in peripheral blood lymphocytes of children with SLE is significantly increased, and the activity of SLE is correlated with the activity of SLE and anti-double stranded DNA antibody in serum.