决明子中8种天然滋味物质的HPLC法同时检测

Determination of Eight Natural Taste Substances in Different Cassia obtusifolia L. Extracts Using HPLC

试验比较不同来源决明子提取物对葡萄糖、果糖、甘露醇、决明子苷、决明子苷Ⅴ、决明子苷Ⅳ、决明子黄素和决明子二醇苯甲酸酯8种天然滋味物质的色谱法检测及决明子来源对其含量的影响。试验采用Waters symmetry C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A相为80%乙腈水溶液(含0.01%三氟乙酸和2.0 mmol/L醋酸铵水溶液);流动相B相为水溶液(含0.01%三氟乙酸和2.0 mmol/L醋酸铵),梯度洗脱,流速为0.8 m L/min,DAD检测波长:254 nm;柱温45℃。检测方法显示:8种天然滋味物质的检测线性范围为0.02~500 mg/L,线性关系良好(r>0.997 0);检出限(S/N=3)在0.005~0.02 mg/kg之间;在加标浓度为1.0~50.0 mg/kg条件下,加标回收率(N=9)在86.34%~103.88%之间,检测方法灵敏有效。

In this study, the influence of different Cassia obtusifolia L. extracts on contents of eight kinds of the natural taste substances （glucose, fructose, mannitol, cassiaside, cassiaside V, cassiaside IV, obtusifolin and Cassia obtusifolia L. benzoic acid esters） in Cassia obmsifolia L. extracts was compared by using HPLC. The experiment was based on Waters symmetry CIs column （250 mm×4.6 mm, 5 μm） with mobile phase A of 80% acetonitrile aqueous solution （containing 0.01% trifluoroacetic acid and 2.0 mmol/L ammonium acetate aqueous solution） and mobile phase B of 0.1% trifluoroacetic acid and 2.0 mmol/L ammonium acetate, and gradient eluted with the flow rate of 0.8 mL/min, DAD detection wavelength of 254 nm and column temperature of 45 ℃. Detection method showed sensitively and effectively： eight kinds of natural taste substances linear range of 0.02-500 mg/L （r〉0.997 0）; The detection limit of between 0.005-0.02 mg/kg （S/N=3）, the recoveries between 86.34%- 103.88% （N=9） in the added concentration range of 1.0-50.0 mg/kg.