东北春麦区小麦品种(系)Bx7亚基超量表达基因(Bx7^(OE))的分子检测

Detection of Overexpressed Bx7 Gene(Bx7^(OE))of Wheat Varieties(Lines)in the Northeast Spring Wheat Region by STS Markers

高分子量谷蛋白亚基Bx7的超量表达可显著提高HMW-GS和不溶性谷蛋白大聚体的含量,使面团强度明显增强。为明确Bx7^(OE)基因在东北春麦区小麦品种(系)中的分布情况,利用2个Bx7^(OE)基因STS标记检测127份东北春麦区小麦品种(系)。结果表明:仅通过选择性回交转Bx7^(OE)基因的克丰6号(7^(OE)+8~*)和龙麦20(7^(OE)+8~*)携带Bx7^(OE)基因。

Due to the over-expression of the high molecular weight glutenin subunit（HMW-GS）Bx7,the content of HMW-GS and insolubility glutenin macro-polymer（GMP）were increased.And the gluten strength also was increased significantly.In order to identify the wheat varieties（lines）carrying Bx7^OE gene in the northeast spring wheat region,127 wheat varieties（lines）were examined by two STS markers.The results showed that only the Kefeng 6 and Longmai20 transferred the Bx7^OE gene by five consecutive backcrosses with biochemical marker assisted selection carried the Bx7^OE gene.