褪黑素对卵母细胞成熟和胚胎体外发育影响的研究进展

Advances in Effects of Melatonin on the Development of Oocytes and Embryos in vitro

卵母细胞与胚胎体外培养过程中,细胞处于相对高氧环境,常常会由于抗氧化防御不足而导致氧化应激,使细胞在发育过程中积累大量的活性氧(ROS),从而降低卵母细胞及胚胎的体外发育率。褪黑素作为一种有效的抗氧化剂和自由基清除剂,能显著降低细胞内ROS水平,清除细胞内自由基,改善卵母细胞的体外成熟与胚胎的体外发育历程。本文从褪黑素对卵母细胞成熟、胚胎体外发育、胚胎质量以及褪黑素作用机制等方面进行综述,为褪黑素在体外胚胎生产中的应用提供参考。

Oxidative stress is often involved in culturing oocytes and embryos in vitro due to a relatively high oxygen environment and inadequate antioxidant defenses. Then large amount of reactive oxygen species （ROS） are accumulated and the rate of oocyte and embryo development in vitro was decreased. As an effective antioxidant and free radical scavenger, melatonin can significantly decrease the intracellular ROS level, remove the intracellular free radicals, and improve the in vitro maturation and in vitro development of oocytes. This review focuses on the effects of melatonin on oocyte maturation, embryo in vitro development, embryo quality and its mechanism in order to further enhance the understanding of melatonin in oocyte and fetal development, and lay the foundation for the application of melatonin in the production of embryos in vitro