ABR反应器处理苯胺黑药废水及其微生物种群结构

Treatment of Aniline Aerofloat Wastewater Using Anaerobic Baffled Reactor and Analysis of Activated Sludge Community

苯胺黑药广泛用作铅锌硫化矿浮选捕收剂,是一种难生化降解浮选药剂.为考察苯胺黑药的厌氧降解行为,采用ABR(厌氧序批式折流板反应器)对苯胺黑药进行处理,利用扫描电镜研究不同隔室中颗粒污泥的性状,并采用Miseq高通量测序的方法分析起主要作用的隔室中的微生物群落组成.结果表明,在进水ρ(苯胺黑药)和ρ(COD_(Cr))有一定波动情况下,苯胺黑药和COD_(Cr)去除率在各阶段均呈增加趋势.100 d成功启动后运行稳定;HRT(水力停留时间)为24 h时,苯胺黑药和COD_(Cr)去除率分别为67%~71%和68%~73%.反应器内出现大量颗粒污泥,沿水流方向颗粒污泥粒径分布逐渐减小,颗粒污泥规则、密实,不会流失.Miseq高通量测序结果表明,反应器第1格和第2格中绿弯菌门菌群所占比例(分别为24.73%和33.60%)最大,其次是厚壁菌门(分别为24.17%和20.92%)和变形菌门(分别为18.93%和20.88%);在属的水平检测到Caldisericum、Leptolinea、Leuconostoc和Bacillus等,使得反应器具有较好的耐冲击负荷和良好的降解作用.研究表明,ABR对苯胺黑药有良好的去除作用.

An anaerobic baffled reactor was developed for the treatment of aniline aerofloat wastewater, a refractory flotation regent. Scanning electron microscopy revealed the shape and properties of granule sludge from different compartments. The microbial communities that might have promary functions were further analyzed by applying Miseq high-throughput sequencing approaches. The results showed that the removal efficiency of aniline aerofloat and C0DCr increased in each stage despite their great fluctuation in the influent. At a hydraulic retention time of 24 h, aniline aerofloat and C0DCr removal reached 67%-71% and 68% - 73% after 100 days＇ operation, respectively. A large amount of granular sludge formed in the reactor, and the particles size distribution was reduced in the direction of flow. The sludge was regular, close-grained and not lost. Miseq high-throughput sequencing results showed that Chloroflexi accounted for most of the sequence （24. 73% and 33. 60% ） , followed by Firmicutes （2 4 . 17% and 20. 92% ） and Proteobacteria （ 18. 93% and 20. 88% ） at the phylum levels. At the genus levels, Caldisericum, Leptolinea, Leuconostoc and Bacillus were iden tified , which meant the reactor had good resistance to impact load and good degradation effect. The study indicated that ABR may be an effective remover of aniline aerofloat.