连翘提取物和连翘挥发油对酵母致热大鼠的解热机制研究

Antipyretic Effect of Forsythia suspense Extract and Forsythia Oil on Yeast-induced Fever Rats and Its Mechanism

对比观察连翘提取物和连翘挥发油对酵母菌所致发热大鼠的解热效应和机制。酵母菌皮下注射复制大鼠发热模型。测基础体温后,随机分为空白组、模型组、连翘提取物组(25生药g/kg)和连翘挥发油组(26.7生药g/kg)四组,动态观察给药后大鼠2 h体温和下丘脑c AMP和PGE2含量。结果表明连翘提取物和连翘挥发油均可显著降低酵母致热大鼠的体温(P<0.01或P<0.05),能显著降低下丘脑中c AMP(P<0.05)含量;另外连翘提取物能显著降低下丘脑中PGE2(P<0.05)含量。连翘提取物有下调下丘脑中c AMP、PGE2作用,连翘挥发油通过下调下丘脑c AMP发挥解热作用,可用于临床发热的对症治疗。

Antipyretic effect and mechanisms of Forsythia suspensa extract and forsythia oil on rats with high fever in-duced by yeast were investigated in the study. Rat model of yeast-induced fever was established by subcutaneous injec-tions of yeast. After the basal body temperature was measured, the rats were randomly divided into four groups ： control group,model group,Forsythia extract group （25 crude drug g/kg） andforsythia oil group （26.7 crude g/kg） . Then the body temperature and the concentration of prostaglandin E2 （ P G E 2 ） and cyclic adenosine monophosphate （ c A M P ） in the hypothalamus cerebrospinal fluid of rats were detected dynamically two hours after dosing. The result showed that F. suspensa extract and forsythia oilcan significantly decrease the temperature （ P 〈0. 01 or P 〈0. 05） as well as hypothala-mus cAMP content（ P 〈0. 01） in yeast-induced fever rats. In addition, F. suspensa extract also obviously decreased hy-pothalamus PGE2 content （ P 〈 0. 01 ） . F. suspensa extract can down-regulate c A M P and P G E 2 in hypothalamus. A n d for-sythia oil down-regulatecAMP in hypothalamus. Both can be used as symptomatic treatment for clinical fever.