miR-218调控SNX4蛋白对乳腺癌细胞增殖和侵袭的影响

Effects of miR-218 on SNX4 protein on proliferation and invasion of breast cancer cells

目的:探讨miR-218调控SNX4蛋白对乳腺癌细胞增殖和侵袭能力的影响。方法:q PCR检测乳腺癌组织和乳腺癌细胞株中miR-218的表达情况;分析miR-218的表达和乳腺癌临床病理参数之间的关系;双荧光素酶实验检测miR-218和SNX4之间的关系;过表达miR-218后MTT实验和侵袭实验检测乳腺癌细胞增殖和侵袭能力变化;过表达SNX4后MTT实验和侵袭实验检测SNX4对乳腺癌细胞增殖和侵袭能力的恢复水平;裸鼠体外成瘤实验检测miR-218对乳腺癌细胞株成瘤能力的影响。结果:miR-218在乳腺癌组织和MCF-7细胞株中表达水平较高,miR-218的表达与乳腺癌的病理分期相关以及淋巴结转移情况有关,SNX4可能是miR-218下游的作用靶点;过表达miR-21可以抑制乳腺癌细胞的增殖侵袭能力,过表达SNX4后可以逆转miR-218对乳腺癌细胞的抑制作用,过表达miR-218后可以抑制乳腺癌细胞株在裸鼠体内的成瘤能力。结论:miR-218在乳腺癌中表达上调,同时miR-218可以调控SNX4的表达而影响乳腺癌细胞增殖和侵袭能力。

ObjectiveTo investigate the effect of miR-218 on the proliferation and invasion of breast cancer cells. Methods： The expression of miR-218 in breast cancer tissues and breast cancer cell lines was detected by qPCR. The relationship between the ex-pression of miR-218 and the clinicopathological parameters of breast cancer were analyzed. Double luciferase assay was used to detect the relationship between miR-218 and SNX4. MTT assay and invasion assay were used to detect the proliferation and invasion of breast cancer cells after overexpression of miR-218. MTX assay and invasion assay were used to detect the recovery level of SNX4 on the pro-liferation and invasion of breast cancer cells. The effect of miR-218 on the tumorigenesis of breast cancer cell lines was examined by tumorigenesis in nude mice. Results ： The expression level of miR-218 in breast cancer tissue and MCF-7 cell line was higher. The expression of miR-218 was associated with pathological stage of breast cancer and lymph node metastasis. SNX4 may be the target of miR-218. Overexpression of miR-21 could inhibit the proliferation and invasion of breast cancer cells. Overexpression of SNX4 could reverse the inhibitory effect of miR-218 on breast cancer cells. Overexpression of miR-218 could inhibit the breast cancer cell line in nude mice tumorigenic ability. Conclusion： miR-218 can up-regulate the expression of miR-218 in breast cancer,and miR-218 can regulate the expression of SNX4 in breast cancer cell proliferation and invasion.