抗SP100抗体和抗GP210抗体在原发性胆汁性肝硬化诊断中的意义

The Significance of Anti-SP100 Antibody and Anti-GP210 Antibody in the Diagnosis of Primary Biliary Cirrhosis

目的探讨抗核体蛋白抗体(抗SP100抗体)和抗核孔膜蛋白抗体(抗GP210抗体)在原发性胆汁性肝硬化(PBC)的诊断价值。方法选择本科收治的128例PBC患者作为病例组,同时选择1 208例非PBC患者作为对照组,采用免疫印迹法分别检测两组患者的抗SP100抗体、抗GP210抗体、抗AMA-M2抗体水平,并计算抗SP100抗体、抗GP210抗体、抗AMA-M2抗体诊断PBC的特异性及敏感性。结果抗SP100抗体、抗GP210抗体、抗AMA-M2抗体检测敏感性分别为27.34%、39.06%、66.41%,特异性分别为99.09%、99.50%、97.76%。结论抗SP100抗体和抗GP210抗体在PBC诊断中均具有较高的特异度,且与抗AMA-M2抗体联合检测可有效提高PBC的检出率。

Objective To evaluate the value of anti-nucleoprotein antibody （anti-SP100 antibody） and anti-nuclear membrane protein antibody （anti-GP210 antibody） in the diagnosis of primary biliary cirrhosis （PBC）. Methods 128 patients with PBC were selected as the case group, and 1 208 patients with non-PBC were selected as the control group. The anti-SP100 antibody, anti-GP210 antibody and anti-AMA-M2 antibody were detected by immunoblotting. And to calculate the specificity and sensitivity of anti-SP100 antibody, anti-GP210 antibody and anti-AMA-M2 antibody in the diagnosis of PBC. Results The sensitivity of anti-SP100 antibody, anti-GP210 antibody and anti-AMA-M2 antibody was 27.34%, 39.06% and 66.41% respectively, and the specificity was 99.09%, 99.50% and 97.76% respectively. Conclusion Anti-SP100 antibody and anti-GP210 antibody have high specificity in the diagnosis of PBC, and combined with anti-AMA-M2 antibody can effectively improve the detection rate of PBC.