宫颈沙眼衣原体和HR-HPV感染与宫颈病变的相关性研究

Study on the Correlation Between Chlamydia Trachomatis and HR-HPV in Cervical Lesion

目的了解宫颈癌、宫颈上皮内瘤变(CIN)患者宫颈分泌物高危型人乳头瘤病毒和沙眼衣原体(CT)感染情况,初步探讨CT、HR-HPV与CIN/宫颈癌的相关性。方法选取2011年9月—2016年12月福建省妇幼保健院因宫颈癌或CIN住院的患者450例设为研究组,另选同期年龄匹配妇科良性疾病患者458例为对照组,采用PCR-反向点杂交技术检测HPV基因分型以及采用免疫层析法检测CT感染情况。结果 HR-HPV在研究组和对照组中感染率分别为88.0%和19.9%,研究组HR-HPV感染率高于对照组,差异有统计学意义(P<0.05);沙眼衣原体在研究组和对照组中感染率分别为19.3%和3.5%,研究组CT感染率高于对照组,差异有统计学意义(P<0.05);以HR-HPV不同感染情况进行分层,HR-HPV阳性或HR-HPV阴性时,研究组CT感染率与对照组对比,差异均具有统计学意义(P<0.05)。结论 CT感染与CIN/宫颈癌有相关性;CT对HR-HPV感染导致的宫颈病变可能存在协同作用。

Objective To explore the relationship of infection of HR-HPV and CT, also the relationship of infection of CT, HR-HPV genotype and CIN or cervical cancer. Methods From September 2011 to December 2016, 450 patients with cervical cancer or CIN hospitalized in Fujian Provincial Maternity and Children Hospital were selected as the study group, 458 cases of age matched gynecologic benign diseases were selected as the control group. PCR- reverse dot blot hybridization was used to detect HPV genotype and CT infection was detected by immunochromatography assay. Results The HR-HPV infection rates in the study and control groups were 88.0% and 19.9%, respectively, the infection rate of HR-HPV in the study group was higher than that in the control group, the difference was statistically significant （P 〈 0.05）. The infection rates of Chlamydia trachomatis in study group and control group were 19.3% and 3.5% respectively, the infection rate of CT in the study group was higher than that in the control group, the difference was statistically signifcant （P 〈 0.05）. Stratify the HR-HPV with different infections, when HR-HPV positive or HR-HPV negative, the CT infection rate in the study group was compared with that of the control group, the differences were statistically signifcant （P 〈 0.05）. Conclusion There may be some relationship between CT positive of cervical intraepithelial neoplasia or cervical cancer. There may be interaction between CT and infection of HR-HPV to cause cervical lesion.