放线菌降解云南昭通褐煤工艺条件优化研究

Optimization of technological conditions for actinomyces degrading Yunnan Zhaotong lignite

对五株放线菌和硝酸氧化的云南昭通褐煤进行菌-煤匹配降解实验,筛选出降解优势菌绿孢链霉菌。在单因素实验的基础上,通过正交实验优化了绿孢链霉菌降解硝酸氧化昭通褐煤的工艺条件。结果表明,影响权重为:煤样粒度>煤浆浓度>接种量>培养时间,最佳工艺条件为:煤样粒度-1 003+500μm,煤浆浓度0.200 0 g/20 m L,接种量1.8 m L/20 m L,培养时间20 d,转化率42.56%。红外分析表明,煤中的有机大分子已被绿孢链霉菌降解成小分子。滤液乙酸乙酯萃取物的GC-MS总离子色谱图证明了降解液中含有丰富的小分子有机物,且大多为脂肪烃、酯、醛、酮、胺及芳香族化合物等。

The degradation matching experiments of Yunnan Zhaotong lignite with five kinds of actinomy-ces had been investigated, showing that Streptomyces viridosporous was the most effective degrading actino-myces. Based on single factor experiments, the process conditions of Streptomyces viridosporous degrading Zhaotong lignite were optimized by orthogonal experiments. The results showed that the effect-weight order of the factors to degradation rate were coal particle size 〉 coal pulp concentration 〉 inoculum size 〉 incu-bation time, the best degrading technological conditions were coal particle size of -1 003 ＋ 500 μm, coal pulp concentration of 0. 200 0 g/20 mL,inoculum size of 1. 8 miy20 mL, incubation time of 20 d. Under this technological conditions, the conversion rate reached 42. 5 6 % . Infrared analysis showed that coal had been disappeared or weakened, which further indicated that organic macromolecular in coal had been de-graded by Streptomyces viridosporous into micromolecule containing in the black oil filtrate derived from the degradation. The total ion chromatogram of ethyl acetate extraction product of degradation filtrate dem-onstrated that it contained abundant small molecule organics, which mostly included aliphatic hydrocar-bons, esters, aldehyde, ketone, amine and aromatic compounds, etc.