依达拉奉注射液对高胆红素血症脑损伤新生大鼠血清S100B蛋白水平的影响

Effects of Edaravone Injection on Serum S100B Protein in Neonatal Rats with Hyperbilirubinemia Induced Brain Injury

目的依达拉奉注射液干预后对高胆红素血症脑损伤新生大鼠血清S100B蛋白浓度的影响。方法将96只7d龄SD大鼠随机分为正常对照组(A组)、高胆红素血症组(B组)、依达拉奉注射液干预组(C组),每组根据处死时间分为4个亚组。给予B组、C组大鼠腹腔注入1次胆红素,同样方法给予A组大鼠注入1次生理盐水,造模后C组大鼠立即腹腔注射依达拉奉注射液,最后在各时间点将各组大鼠血清及脑组织取出。采用HE染色法观察脑组织的病理学改变,Elisa法测定血清S100B蛋白浓度,计算相关数据并进行统计学分析。结果造模后,明显的神经行为异常均出现在B组和C组大鼠,各时间点C组大鼠较B组症状明显改善,A组大鼠行为无明显异常。造模24h后,B组、C组大鼠脑组织出现水平不一的水肿、变性及炎性浸润等,随着时间延长程度加重。各时间点C组大鼠脑组织改变程度较B组减轻,A组大鼠脑组织结构基本正常。B组大鼠血清S100B蛋白浓度高于A组、C组,与时间呈正相关,差异有统计学意义(P<0.05);C组大鼠血清S100B蛋白浓度低于B组,高于A组,差异有统计学意义(P<0.05)。结论经依达拉奉注射液干预后,不同时间点脑组织改变不同程度减轻,高胆红素血症大鼠血清S100B蛋白浓度明显降低,表明依达拉奉注射液可减轻过量胆红素对中枢神经系统的损伤,起到保护神经元作用。

Objective To explore the effects of edaravone injection on serum S100 Bprotein in neonatal rats with hyperbilirubinemia induced brain injury.Methods Ninety-six seven-day-old Wistar Sprague-Dawley（SD）rats were randomly assigned into three groups：normal control group（group A）,hyperbilirubinemia group（group B）,and edaravone injection group（group C）.Then each group was divided into 4 subgroups based on the time of execution.The rats in group B and group C were injected intraperitoneally with bilirubin.The rats in group A were injected intraperitoneally with normal saline once.After modeling,rats in group C were given intraperitoneal injection of edaravone injection.The serum and brain tissue were taken out at different time points.The pathological changes of brain tissue were observed by HE staining.The concentration of serum S100 B protein was measured by Elisa method.Results After modeling,neurological abnormalities were observed in group B and group C.The symptoms of rats in group C had significantly improved compared with group B at each time point.After 24 hours of modeling,the edema,degeneration and inflammatory infiltration of brain tissue in group B and group C were aggravated with the prolongation of time.The changes of brain tissue in group C were lower than those in group B at each time point.The structure of brain tissue in group A was normal.The concentration of S100 B protein in group B was higher than that in group A and group C,and positively correlated with time（P〈0.05）.The concentration of serum S100 B protein in group C was lower than that in group B（P〈0.05）.Conclusion After intervention with edaravone injection,the changes of brain tissue in the hyperbilirubinemia rats at different time points were reduced to different degrees.The concentration of S100 B protein was significantly decreased at different time points.The results indicated that edaravone injection could reduce the damage of excess bilirubin on central nervous system,and play a role in protecting neurons.