喘息性肺炎患儿全血中T淋巴细胞亚群和自然杀伤细胞的表达研究

Study on the peripheral blood T lymphocyte subsets and natural killer cell in children with asthmatic pneumonia

目的探讨喘息性肺炎患儿全血中T淋巴细胞亚群和自然杀伤细胞的表达及意义。方法选取2013-2016年在中山市人民医院确诊为喘息性肺炎患儿36例作为喘息性肺炎组,另选取同期来该院体检健康儿童36例作为对照组,采用流式细胞仪检测分析两组全血中T淋巴细胞亚群和自然杀伤细胞的表达,并做ROC曲线进行结果分析。结果喘息性肺炎组CD3^+T淋巴细胞、CD3^+CD8^+T淋巴细胞、CD28^+T淋巴细胞、CD8^+CD28^+T淋巴细胞、CD8^+CD28-T淋巴细胞、CD3-CD16^+CD56^+自然杀伤细胞、CD3^+CD16^+CD56^+自然杀伤细胞、CD3^+CD4^+/CD3^+CD8^+与对照组比较,差异有统计学意义(P<0.05)。ROC曲线统计结果显示,流式细胞仪检测T淋巴细胞亚群和自然杀伤细胞各参数均有较高的灵敏度。结论喘息性肺炎患儿存在免疫功能障碍,免疫功能的改变与喘息性肺炎发生相关,对喘息性肺炎患儿进行免疫功能检测具有重要的临床意义。

Objective To explore the expression and significance of peripheral blood T lymphocyte subgroups and natural killer cell in children with asthmatic pneumonia.Methods From 2013 to 2016,asthmatic children with pneumonia were erolled in the study as asthmatic pneumonia group(36cases)and 36 cases medical health children as control group at the same time in Zhongshan City people′s hospital.Flow cytometry was used to test analysis of two groups of T lymphocyte subsets and natural killer cell in serum and the expression of CD28,CD56,using ROC curve analysis results.Results Asthmatic pneumonia group compared with control group,the difference of CD3^+ and CD3^+CD8^+,CD28^+,CD8^+CD28^+,CD8^+CD28-,CD3-CD16^+CD56^+and CD3^+CD16^+CD56^+,CD3^+CD4^+/CD3^+CD8^+was statistically significant(P<0.05).According to the results of flow parameters have higher sensitivity by the ROC curve statistics.Conclusion Asthmatic pneumonia children have immune dysfunction,immune function changes associate with asthmatic,which is of great clinical significance to carry out immune function detection for children with asthmatic pneumonia.