封闭式负压引流技术对人慢性创面血管生成的作用研究

Study on the effect of closed negative pressure drainage technology on the formation of chronic wound blood vessels

目的研究封闭式负压引流对人慢性创面血管生成的影响作用。方法以该院在2015年11月至2016年11月收治的慢性创面患者12例，均行封闭式负压引流治疗，于吸引前、吸引后l、3、5、7d分别取其创缘组织，借助于免疫组织化学技术的应用，对其血管生成的作用进行研究。结果在封闭式负压引流治疗之前，患者创缘组织真皮浅层血管内皮细胞可少见有VEGF阳性表达，基于治疗时间的延长，创缘组织真皮层血管内皮细胞、细胞VEGF阳性表达显著增强，标记指数显著增多（P〈0．05）；在CD34免疫组化方面来看，治疗后，所表达的微血管密度显著增强（P〈0．05）。结论封闭式负压引流技术促进慢性创面肉芽组织的生长，对慢性创面患者的治疗有积极影响作用。

Objective to study the effect of closed negative pressure drainage on human chronic wound angiogenesis. Methods our hospital in November 2015 to November 2016 were 12 cases of patients with chronic wounds as the research object, all closed negative pressure drainage treatment,before the draw,draw after 1 d,3 d, 5 d, 7 d respectively take its edge organization, with the aid of the application of the immunohistochemical technique,to study the role of angiogenesis. Results in patients with closed nega- tive pressure drainage before treatment, the edge tissue dermal shallow vascular endothelial cells can be rare VEGF positive expres- sion,based on the extension of treatment time,the edge tissue dermis vascular endothelial cells, cells significantly enhanced VEGF positive expression, tag index significantly increased（P〈0.05） ;In the case of CD34 immunohistochemistry,the microvascular den- sity expressed in the treatment was significantly enhanced（P〈0.05）. Conclusion closed negative pressure drainage technology promotes the growth of chronic wound granulation tissue,and has positive effect on the treatment of chronic wound.