西藏林芝地区仔猪白痢血清型及毒力基因检测

Serotype test and virulence genes detection of Tibetan piglets with white score diarrhea in Tibet

为探究西藏林芝地区仔猪白痢大肠杆菌优势血清型和毒力基因间的相关性,采用玻片凝集试验测定与仔猪白痢有关的血清型,PCR方法调查11种毒力基因。结果显示,在分离的81株藏猪源大肠杆菌里有72株检测到毒力基因,其中黏附素K88和CS31A分别检测到1(1.39%)和34(47.22%)株;肠毒素STa、STb和EAST1分别检测到10(13.89%),16(22.22%)和38(52.78%)株;毒力岛eaeA、irp2和ETT2分别检测到12(16.57%),44(61.11%)和56(77.78%)株。72株中有59株鉴定出9种血清型,其中有15株定型为O8(20.99%),11株定型为O64(16.05%),10株定型为O138(13.58%)。研究表明:O8、O64、O138为优势血清型,且O8与STa+STb相关,O107与肠毒素STa相关,O64与毒力岛eaeA+ETT2有关,毒力基因以EAST1+CS31A+irp2+ETT2组合检出率最高为18(25%)株。

To explore the correlation of preponderant Escherichia coli （ E. coli ） serotypes and viru- lence genes of Tibetan piglets with white score diarrhea,slide agglutination and PCR method were used to determine the related serotypes and 11 virulence genes, respectively. The results showed that 72 strains with virulence genes were isolated from 81 strains isolated from Tibetan piglets, ad- hesin K88 and CS31A were detected 1 （1.39%） and 34 （47.22%）,respectively;enterotoxin STa, STb and EAST1 were recovered 10 （13.89%）,16 （22.22%） and 38 （52.78%）,respectively;path- ogenicity island eaeA, irp2 and ETT2 were detected 12 （16. 57%）, 44 （61. 11%） and 56 （77.78%） ,respectively. Nine serotypes were recovered from the 59/72 strains with virulence genes,including 15 （20.99%） O8,11 （16.05%） 064 and 10 （13.58%） O138 serotypes,respec- tively. This study showed that 08,064 and O138 are the dominant serotypes,and STa ＋ STb is related to 08, O107 is relevant to enterotoxin STa,pathogenicity island eaeA ＋ ETT2 is related to O64,and the combination of EAST1,CS31A,irp2 and ETT2 virulence genes was tested in 18 iso- lates （25%）,which is the highest detection rate.