山东省鸡源致病性大肠杆菌O-抗原混合血清型分析

Analysis of mixed O-antigen serotypes of E.coli isolated from chickens in Shandong province

采用抗原抗体凝集反应,用常见的18种O因子血清测定从临床上分离鉴定的182株大肠杆菌的O抗原血清型。结果显示,山东地区鸡源大肠杆菌的优势血清型为O24,O15,O2,O127,O1,O18,O78,占检测菌株的41.21%,同时出现大量混合血清型,占检测菌株的24.18%。这是国内首次报道检测到大量的大肠杆菌O因子混合血清型,印证了国外研究人员对O-AGC核苷酸序列研究的结果,有助于最终对O抗原血清型进行更加准确的分类以及大肠杆菌疫苗的研制。

The O-antigen serotypes of 182 strains of E. coil,which were isolated and identified from chicken of different districts in Shandong province,were tested against 18 kinds of O factor serum by antigen-antibody agglutination reacting. The results shows the dominant serotypes of Esche- richia coli in Shandong province are O24, O15,02, O127, O1, O18 and 078 respectively, which ac- counts for 41.21% ,and displayed a lot of mixed O-antigen serotypes which accounts for 24.18% of tested strains. It is first reported that there are many mixed O-antigen serotypes of Escherichia coli in China,which are in accord with previous reports from foreign.