摘要
在单因素试验基础上,通过正交试验初步优化确定了西兰花中类胡萝卜素提取的最佳工艺条件为:用无水乙醇作为提取液、料液比1∶60、提取时间180min、提取温度55℃,提取率可达268.26mg/100g。同时通过水杨酸法测定类胡萝卜素粗提物清除羟基自由基的能力,证实西兰花类胡萝卜素具有较强的抗氧化能力。
The optimal conditions of extraction carotenoids,including solvent,ratio of solid to liquid,temperature,time,and extracting frequencies,were determined based on single factor analysis,then orthogonal experiment was performed to determine the optimum extraction conditions. The extraction rate reached the highest value as 268. 26mg/100 g,when the broccoli powder was soaked by ethanol with ratio of solid to liquid being 1∶60 for 180 min under 55℃. Mainly determination on hydroxyl radical scavenging ability of crude extracts by salicylic acid method proved the carotenoid crude extract had strong antioxidant capacity.
作者
李世红
李媛媛
张佳怡
余威
陈文玉
苏金芬
王岁楼
QU Hui-ming CHU Jie HAN Li-wen LIU Jian-long LIU Ke-chun(Life Sciences Institute of Shandong Normal University, Jinan 250014, China Biology Research Institute of Shandong Academy of Sciences/Shandong Provincial Engineering Laboratory for Biological Testing Technology/Key laboratory for Biosensor of Shandong Province/Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Jinan 250014, China)
出处
《中国食物与营养》
2017年第8期42-44,共3页
Food and Nutrition in China
基金
2016年国家级大学生创新创业训练计划项目(项目编号:J1310032)
关键词
西兰花
类胡萝卜素
提取工艺
抗氧化能力
broccoli
carotenoids
extraction condition
antioxidant capacity
