Wnt5b在乙型肝炎相关肝细胞癌中的表达及意义

Expression of Wnt5b in patients with HBV-related hepatocellular carcinoma and its clinical significance

目的观察Wnt5b在乙肝相关肝细胞癌患者中的表达情况及其与患者临床病理参数间的关系。方法采用简单随机抽样的方法从289例乙肝相关肝细胞癌患者中抽取两组(各100例)患者,采用实时荧光定量PCR(q RT-PCR)和免疫组织化学染色分别检测两组患者肿瘤和非癌组织中Wnt5b mRNA和蛋白的表达水平,并分别统计分析它们与患者临床病理参数及预后间的关系。结果 Wnt5b mRNA在癌组织中普遍较非癌组织高表达(65.0%,65/100),Wnt5b蛋白在癌组织中的表达阳性率明显高于非癌组织(58.0%vs 22.0%,P<0.05),且Wnt5b的表达水平均与肿瘤大小(P<0.05)、数目(P<0.01,仅蛋白水平)、分化程度(P<0.01,仅蛋白水平)、TNM分期(P<0.05)、巴塞罗那分期(P<0.05)、转移(P<0.05)、复发(P<0.01)等临床病理参数显著相关;高表达Wnt5b mRNA和蛋白的患者术后无瘤生存时间更短(P<0.01)。结论 Wnt5b的高表达参与HCC的进展并提示患者预后不良。

Objective To investigate the expression of Wnt5b in hepatitis B virus （HBV）-related hepatocellular carcinoma （HCC） tissues and its correlation with the clinicopathological parameters. Methods Quantitative real-time polymerase chain reaction （qRT-PCR） and immunohistochemical staining were employed to measure Wnt5b mRNA and protein expressions in two groups of HBV-related HCC patients （100 cases in each） selected from a cohort of 289 cases with HBV-related HCC using simple random sampling method. The correlation of Wnt5b expression with the clinicopathological parameters and the prognosis of HCC patients was analyzed. Results Wnt5b mRNA expression was significantly higher in HCC tissues than that of adjacent noncancerous tissues in 65.0% （65/100） of the cases, and the positivity rate of Wnt5b protein was significantly higher in HCC tissues than that of adjacent noncancerous tissues （58.0%vs 22.0%, P〈0.05）. Wnt5b expression was significantly correlated with the tumor size （P〈0.05）, tumor number （P〈0.01, only at the protein level）, tumor differentiation （P〈0.01, only at the protein level）, TNM stage （P〈0.05）, BCLC stage （P〈0.05）, metastasis （P〈0.05） and recurrence （P〈0.01）. The patients with up-regulated Wnt5b mRNA and protein had a shorter relapse-free survival （P〈0.01）.Conclusions Up-regulated Wnt5b might contribute to the progression of HBV-related HCC and predicts a poor prognosis.