槲皮素PLGA-TPGS纳米粒对小鼠肝癌异位实体瘤治疗效果的研究

The Therapeutic Effect of Quercetin-loaded PLGA-TPGS Nanoparticles on the Hepatocarcinoma Ectopic Solid Tumor-bearing Mice

目的考察槲皮素PLGA-TPGS纳米粒(QPTN)在体内对荷腹水型肝癌高淋巴道转移细胞HCa-F小鼠异位移植实体瘤的治疗效果。方法建立荷HCa-F肝癌细胞小鼠模型后,随机分为阴性对照组、空白纳米粒组、5-氟尿嘧啶溶液(FS)组、槲皮素溶液(QTS)组、槲皮素PLGA纳米粒(QPN)组和QPTN组。尾静脉给药,每2 d 1次,连续给药20 d后处死小鼠,剥离肿瘤,称质量,测量肿瘤体积,根据公式计算肿瘤体积增长量和抑瘤率;行HE染色观察肿瘤,全面评价QPTN对荷瘤小鼠的治疗效果。结果小鼠体内给药10次后,QPTN组、QPN组、FS组的肿瘤体积增长量与阴性对照组相比明显减小(P<0.05或P<0.01),QPTN组抑瘤率(59.07%)明显高于QTS组(23.94%)、FS组(35.14%)和QPN组(46.14%)。HE染色结果也显示QPTN组对小鼠肿瘤的治疗效果最明显。结论与QPN、QTS和FS相比,QPTN对荷HCa-F肝癌细胞小鼠异位实体瘤具有较好的治疗效果。

Objective To investigate the therapeutic effects of Quercetin （QT）-loaded PLGA-TPGS nanoparticles （QPTN） on solid tumor-bear- ing mice with HCa-F hepatocarcinoma in vivo. Methods The model of HCa-F hepatocarcinoma solid tumor-bearing mice was established by im- planting HCa-F cells into 48 mice. The mice were divided into 6 groups randomly ： the negative control, empty PLGA-TPGS nanoparticles, 5-Fluo- roumcil solutions （FS）, Quercetin solutions （QTS）, QT-loaded PLGA nanoparticles （QPN）, and QPTN groups. Each group was treated using tail vein twice a day for 20 days ; then, all mice were sacrificed. The increment tumor volumes and tumor growth inhibition rate were counted. Then, tumor specimens were prepared for hematoxyhn ＆ eosin （HE） staining and observed under a microscope. Results The results showed that the increment tumor volumes of mice in the QPTN, QPN, and FS groups were significantly smaller than that in the negative control group （P 〈 0.05 or P 〈 0.01 ）. The tumor growth inhibition rate of the QPTN group was 59.07%, which was much higher than that of the QTS group （23.94%）, the FS group （35.14%）, and the QPN group （46.14%）. The results of the HE staining on the tumor sections also indicated that the QPTN group showed a better therapeutic outcome compared to the other groups. Conclusion The QPTN has a better therapeutic effect on the model of solid tumor using HCa-F cells-bearing mice than the QPN, QTS, and FS.