番茄裂果性状及QTL定位分析

Fruit Cracking and QTL Mapping of Tomato

以耐裂果的加工番茄‘14803’和易裂果的栽培番茄‘14633’为试材,将二者杂交得到F_1代,F_1自交得到F2,研究了F_2子代的裂果相关性及QTL定位,以期为近一步培育耐裂果、品质优良的新品种提供参考依据。结果表明:番茄果实抗裂指数与果实纵径、果形指数、果肩果皮韧度、果腹果皮韧度4个指标呈显著正相关。通过利用SSR和AFLP分子标记对F_2群体进行QTL定位及遗传连锁图谱的构建,找到了27个与番茄果质量、果形指数、果皮韧度等相关的QTLs,5个抗裂指数相关的QTLs。

Resistant crack tomato ‘14803’ and easily crack tomato ‘14633’ were used as test materials,the F1 generation was obtained by hybridization of ‘14803’ and ‘14633’,the resulting F1 plants were self-crossed to obtain F2.Correlations among fruit cracking and QTL of the F2 generation were investigated in this study,in order to provide a theoretical basis for further cultivating new varieties with crack resistance and good quality.The results showed that there were significant positive correlations between the crack resistance index and fruit longitudinal diameter,fruit shape index,the toughness of the peel on the fruit shoulder and the toughness of the peel in the middle of the fruit.The SSR and AFLP techniques were used to construct the genetic linkage map and QTL mapping.A total of 27 QTLs related to fruit weight,fruit shape index and fruit toughness were detected by F2 generation,among them there were 5 QTLs related with crack resistance index.