芽孢杆菌BCJB01和BMJBN02对葡萄霜霉病的田间防效

Efficacy of Bacillus spp.BCJB01 and BMJBN02 on Grape Downy Mildew

以葡萄霜霉病生防菌株BCJB01和BMJBN02为供试菌,分别在山东省济南和烟台地区的葡萄园对不同葡萄品种的霜霉病进行了小区防治试验,以期明确BCJB01和BMJBN02对不同葡萄品种霜霉病的实际防治效果,探寻提高BCJB01和BMJBN02菌剂对葡萄霜霉病的防治技术,为其制剂的推广应用提供参考依据。结果表明:在霜霉病发病初期,20mL·L^(-1)芽孢杆菌BCJB01和BMJBN02悬浮剂对"泽山一号"的防治效果分别为84.05%和73.30%;20mL·L^(-1)芽孢杆菌BCJB01和BMJBN02悬浮剂交替使用对"蛇龙珠"的防效在62%以上;20 000 mg·L^(-1)?BCJB01粉剂+0.1%助剂对"蛇龙珠"的防效为81.31%;6 667mg·L^(-1)?BCJB01粉剂+0.1%助剂对"蛇龙珠"的防效为61.29%。在霜霉病进入盛发期后,20 000mg·L^(-1)BCJB01粉剂+0.1%助剂对"蛇龙珠"的防效仍在80%以上;6 667mg·L^(-1)芽孢杆菌BCJB01粉剂对"夏黑"的防治效果为62.65%。表明BCJB01和BMJBN02悬浮剂及BCJB01粉剂+0.1%助剂对葡萄霜霉病均有良好的防效,可作为防治葡萄霜霉病的交替生物药剂。

Biocontrol strains BCJB01 and BMJBN02 were used as test bacteria,the field efficacy trials of grape downy mildew were studied in Shandong Province,Jinan and Yantai vineyards respectively,to defining the effects of BCJB01 and BMJBN02 on the control of downy mildew of different grape varieties,and exploring the control techniques,provide the theoretical basis for the popularization and application BCJB01 and BMJBN02 preparations.The results showed that the control effects of 20 mL·L-1 BCJB01 bacillus and BMJBN02 suspending agent on ‘Zeshan No.1’ were 84.05% and 73.30% respectively at the beginning of the grape downy mildew.The control effects of 20 mL·L-1 BCJB01 bacillus and BMJBN02 suspending agent on ‘Cabernet Gernischt’ was above 62% when they could be used in alternation.The control effects of 20 000 mg·L-1 BCJB01 soluble powder and 0.1% assistant on ‘Cabernet Gernischt’ was 81.31% while 6 667 mg·L-1 BCJB01 soluble powder and 0.1% assistant reduced disease severity to 61.29%.In the full incidence period of downy mildew,the control effects of 20 000 mg·L-1 BCJB01 soluble powder and 0.1% assistant on ‘Cabernet Gernischt’ was 81.31%.It was effective on controlling ‘Summber black’ grape downy mildew by using 6 667 mg·L-1 BCJB01 soluble powder （62.65%）.The results showed that alternate using BCJB01 and BMJBN02 SC or using BCJB01 WP added 0.1% additives,all had good control effects on grape downy mildew,and they were good alternative fungicides to grape downy mildew.