低温胁迫下人参愈伤组织中氧化鲨烯环化酶基因家族的表达特性分析

Effect of Chilling Stress on Expression Characteristic of Oxidosqualene Cyclase (OSC) in Callus of Panax ginseng

以培养3周的新鲜人参愈伤组织为试材,分别进行5℃持续低温处理与25℃/5℃培养12h/12h间歇低温处理,取材时间为0、1、2、3、4d,分别设为CK、D1、D2、D3、D4处理,通过实时荧光定量PCR检测5种氧化鲨烯环化酶(OSC)基因在不同处理中的表达。结果表明:PgDAS的表达在持续式低温胁迫D4处理和间歇式低温胁迫D3处理中被显著诱导并达到峰值,分别为对照组的2.15、2.12倍;而Pgβ-AS1与Pgβ-AS2的表达仅在持续式低温胁迫D2处理中被诱导,分别为对照组的1.27、1.71倍;PgCAS与PgLOS的表达在2种低温胁迫中均未被诱导。表明PgDAS可能在响应不同低温胁迫时起到关键作用,而其它氧化鲨烯环化酶基因协同表达。

The continuous （5 ℃） and intermittent （25 ℃/5 ℃, 12 h/12 h） chilling treatments were performed by three weeks fresh callus,which were harvested after 0,1,2,3 and 4 days treatment and recorded as CK,D1,D2,D3 and D4, respectively, the expressions of five kinds of oxidosqualene cyclase （OSC） genes in different treatments were detected by real-time quantitative PCR. The results showed that the expression of PgDAS was significantly induced in the 134 of continuous chilling treatment and D3 of intermittent chilling treatment, which were 2.15 times and 2.12 times of the control group, respectively;whereas Pgfl-AS1 and Pgfl-AS2 expression were only induced in the D2 of continuous chilling stress treatment, which were 1.27 times and 1.71 times of the control group, respectively; PgCAS and PgLOS expression were not induced in both two kinds of chilling stress treatments. It indicated that the PgDAS maight play a key role in response to different chilling stress treatments, and the other OSC genes might be synergistic expression with PgDAS.