承德两种野生食用菌的分子鉴定及系统发育分析

Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area

以承德红蘑和白蘑子实体为试材,采用真菌通用引物ITS1和ITS4,对其基因组rDNA基因的ITS区段进行PCR扩增、克隆测序,而后与GenBank数据库信息比对,并利用MEGA 5.0软件构建系统发育树,从分子水平上鉴定其种属地位及系统进化关系。结果表明:承德红蘑与血红铆钉菇(Chroogomphus rutilus,Accession No.:KR082876)、白蘑与Hygrophorus queletii(Accession No.:JF908069)的相似性均达到99%。承德红蘑与血红铆钉菇C.rutilus(Accession No.:KR082876),承德白蘑与H.queletii(Accession No.:JF908069)的遗传关系最近,遗传距离为0.002。因此,承德红蘑和白蘑经分子鉴定为C.rutilus和H.queletii,其分类地位的解析,对今后野生红蘑和白蘑种质资源鉴定、生物学特性研究及开发利用奠定了基础。

In order to elucidate taxonomic status and phylogenetic relationship of wild red mushroom and white mushroom in Chengde area,the ITS regions of rDNA genes were amplified using ITS1 and ITS4 as primers, cloned and sequenced; ITS sequences were then analyzed via NCBI blast program, meanwhile phylogenetic tree were constructed using MEGA 5.0 software, respectively. The results indicated that the similarity of between red mushroom from Chengde area and Chroogornphus rutilus （Accession No. ： KR082876）was up to 99%, and genetic distance was 0. 002. White mushroom,which was another kind of precious wild edible fungi in Chengde area, also had 99% similarity with Hygrophorus queletii （Accession No.： JF908069）, and their genetic distance reached to 0. 002. Therefore,red mushroom and white mushroom in Chengde area were at the molecular level identified as C. rutilus and H. queletii, respectively. The elucidation of their taxonomic status would be beneficial for identification, biological characteristics, and development and utilization of wild red mushroom and white mushroom.