急性肺损伤小鼠肺泡巨噬细胞TREM1 mRNA的表达及意义

Expression and Clinical Significant of TREM1 mRNA of Rat Alveolar Macrophages in Acute Lung Injury

目的:研究脂多糖(LPS)致急性肺损伤(ALI)小鼠肺泡巨噬细胞(AM)髓系细胞触发受体1(TREM1)mRNA的表达及意义。方法:48只小鼠分成对照组和实验组,对照组腹腔注射生理盐水,实验组腹腔注射LPS诱导ALI模型;于灌注后3、6、12及24 h,采用荧光定量逆转录(RT-PCR)方法检测支气管肺泡灌洗液中提取的AM中TREM1 mRNA表达水平,用ELISA法检测支气管肺泡灌洗液上清液中的TNFα、可溶性TREM1(sTREM1)水平,并分析TREM1 mRNA与sTREM1和TNFα表达水平的相关性。结果:各对照组AM均表达TREM1 mRNA,但各时间点比较差异无统计学意义(P>0.05);观察组TREM1 mRNA、TNFα及sTREM1的表达均在3 h时上升致最高,之后下降;TREM1mRNA与sTREM1及TNFα的表达水平呈正相关(r=0.783,P<0.001;r=0.472,P<0.05)。结论:ALI小鼠支气管肺泡灌洗液、及其AM中TREM1 mRNA表达水平升高,TREM1 mRNA升高与sTREM1、TNFα呈正相关。

Objective： To investigate the expression and clinical significance of TREM1 mRNA in the alveolar macrophages（ AM） of acute lung injury induced by lipopolysaccharide（ LPS） in rat. Methods： 48 rats were randomly divided into control group and experiment group,control group was intraperitoneally injected with normal saline; experiment group was intraperitoneally injected with LPS to induce ALI model. After 3,6,12 and 24 h,RT-PCR was adopted to detect TREM1 mRNA expression level in AM extracted from bronchoalveolar lavage fluid. ELISA was used to detect the concentration of TNF-α and soluble TREM1 level in supernatant of bronchoalveolar lavage fluid; analyzing correlation between expression level of TREM1 mRNA and sTREM1 together with TNFα. Results： AM of control group expressed TREM1 mRNA,while comparison differences of each time point showed no statistical significance（ P〉0. 05）; the expression of TREM1 mRNA,TNFα and sTREM1 rose to the highest in 3 h,then declined; expression of TREM1 mRNA and TNFα and sTREM1 level were positively correlated（ r = 0. 783,P〈0. 001 and r = 0. 472,P〈0. 05）. Conclusion： ALI rat bronchoalveolar lavage fluid and expression level of TREM1 mRNA increased,the increase of TREM1 mRNA was positively correlated with sTREM1 and TNFα.