双酚A(BPA)对人原代绒毛外滋养细胞(EVT)迁移和侵袭能力的影响

Effect of bisphenol A(BPA) on migration and invasion of extravillous trophoblast(EVT) cells

目的:研究双酚A(bisphenol A,BPA)对绒毛外滋养细胞(extravillous trophoblasts,EVT)迁移和侵袭能力的影响。方法:本研究共收集98例胎盘绒毛标本,采用随机分组的方式将EVT细胞分为对照组和不同浓度的BPA处理组(每组≥3例)。用免疫荧光法(immunofluorescence,IFC)对分离的对照组EVT细胞进行鉴定;BPA暴露48 h后,用流式细胞术(FCM)检测BPA暴露对EVT细胞活力、凋亡和增殖的影响;用Transwell法检测EVT细胞的迁移和侵袭能力。结果:(1)IFC显示EVT细胞中人类白细胞抗原G(human leukocyte antigen G,HLA-G)阳性细胞的比例即EVT细胞的纯度>92.7%;(2)流式细胞术和免疫印迹法显示非毒性剂量的BPA不影响EVT细胞的凋亡(F=1.808,P=0.233)、活力(F=0.624,P=0.609)和增殖能力(F=0.174,P=0.913;F=0.260,P=0.852);(3)Transwell显示EVT的迁移和侵袭能力随BPA浓度的增高而呈剂量依赖性增强(F=24.080,P=0.000;F=144.900,P=0.000)。结论:BPA以剂量依赖的方式增强EVT细胞的迁移和侵袭能力。

Objective：To study the effect of bisphenol A（BPA）on the migration and invasion abilities of extravillous trophoblasts（EVT）. Methods：In this study,98 placental villi were collected and randomly divided into control group and BPA treatment groups（more than 3 cases in each group）. Immunofluorescence（IFC）was used to identify the purity of control EVT cells;Flow cytology（FCM）was used to test the cell viability,apoptosis and proliferation ability of EVT after BPA exposure for 48 h. Transwell assay was used to measure the migration and invasion ability of EVT cells. Results：（1）IFC assay showed that the purity of EVT（human leukocyte antigen G,HLA-G positive cells）was above 92.7%.（2）FCM and Western blot assays indicated that cell apoptosis（F=1.808,P=0.233）,viability（F=0.624,P=0.609）and proliferation（F=0.174,P=0.913;F=0.260,P=0.852）ability of EVT were not affected by the non-toxic doses of BPA.（3）BPA increased the migration and invasion ability of EVT in a dose-dependent manner（F=24.080,P=0.000;F=144.900,P=0.000）. Conclusion：BPA increased the migration invasion abilities of EVT cells in a dose-dependent manner.